Chromatin immunoprecipitation (ChIP) exploits the specific interactions between DNA and DNA-associated proteins. It can be used to examine a wide range of experimental parameters. A number of proteins bound at the same genomic location can identify a multi-protein chromatin complex where several proteins work together to regulate gene transcription or chromatin configuration. In many instances, this can be achieved using sequential ChIP; or simply, ChIP-re-ChIP. Whether it is for the examination of specific transcriptional or epigenetic regulators, or for the identification of cistromes, the ability to perform a sequential ChIP adds a higher level of power and definition to these analyses. In this chapter, we describe a simple and reliable method for the sequential ChIP assay.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-7380-4_9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An integrated paper-based microfluidic platform for screening of early-stage Alzheimer's disease by detecting Aβ42.
Lab Chip
January 2025
School of Advanced Technology, Xi'an Jiaotong-Liverpool University, Suzhou, 215000, China.
Alzheimer's disease (AD) is the leading cause of dementia worldwide, and the development of early screening methods can address its significant health and social consequences. In this paper, we present a rotary-valve assisted paper-based immunoassay device (RAPID) for early screening of AD, featuring a highly integrated on-chip rotary micro-valve that enables fully automated and efficient detection of the AD biomarker (amyloid beta 42, Aβ42) in artificial plasma. The microfluidic paper-based analytical device (μPAD) of the RAPID pre-stores the required assay reagents on a μPAD and automatically controls the liquid flow through a single valve.
View Article and Find Full Text PDFTime-resolved single-cell secretion analysis microfluidics.
Lab Chip
January 2025
Department of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon Tong, Hong Kong, China.
Revealing how individual cells alter their secretions over time is crucial for understanding their responses to environmental changes. Key questions include: When do cells modify their functions and states? What transitions occur? Insights into the kinetic secretion trajectories of various cell types are essential for unraveling complex biological systems. This review highlights seven microfluidic technologies for time-resolved single-cell secretion analysis: 1.
View Article and Find Full Text PDFGenomic prediction accounting for dominance and epistatic genetic effects on litter size traits in Large White pigs.
J Anim Sci
January 2025
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, PR China.
Litter size traits of sows are crucial for the economic benefits of the pig industry. Three phenotypic traits of 1,206 Large White (LW) pigs, that is, the total number born (TNB), number born alive (NBA), and number of healthy piglets (NHP), were recorded. We evaluated a series of genomic best linear unbiased prediction (GBLUP) models that sequentially added additive effects (model A), dominance effects (model A+D), and epistatic effects (model A+D+AA, model A+D+AA+AD, and model A+D+AA+AD+DD) using chip data and imputed whole-genome sequencing (WGS) data to estimate genetic parameters and predictive accuracy.
View Article and Find Full Text PDFAn integrated magnetoimpedance biosensor microfluidic magnetic platform for the evaluation of the cardiac marker cTnI.
Anal Methods
January 2025
Microelectronic Research & Development Center, School of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, China.
An integrated magnetoimpedance (MI) biosensor microfluidic magnetic platform was proposed for the evaluation of the cardiac marker, cardiac troponin I (cTnI). This bioanalyte evaluation platform mainly comprised three external permanent magnets (PMs), one MI element, two peelable SiO film units and a microfluidic chip (MFC). The MI element was made of micro-electro-mechanical system (MEMS)-based multilayered [Ti (6 nm)/FeNi (100 nm)]/Cu (400 nm)/[Ti (6 nm)/FeNi (100 nm)] thin films and designed as meander structures with closed magnetic flux.
View Article and Find Full Text PDFHigh-throughput, combinatorial droplet generation by sequential spraying.
Lab Chip
January 2025
Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York, USA.
Advancements in bulk and microfluidic emulsion methodologies have enabled highly efficient, high-throughput implementations of biochemical assays. Spray-based techniques offer rapid generation, droplet immobilization, and accessibility, but remain relatively underutilized, likely because they result in random and polydisperse droplets. However, the polydisperse characteristic can be leveraged; at sufficiently high droplet numbers, sequential sprays will generate mixed droplets which effectively populate a combinatorial space.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!