AI Article Synopsis
- The study investigated how theanine content in tea leaves changes after harvesting under different temperature and shading conditions using advanced liquid chromatography.
- Differentially expressed proteins, CsFd-GOGAT and CsNADH-GOGAT, were linked to theanine production, with their interactions confirmed through yeast two-hybrid technology.
- The research also analyzed the expression of 17 relevant genes during theanine metabolism and found that CsGOGAT plays a significant role in the dynamic changes of theanine content.
Article Abstract
We analyzed the changes of theanine content in postharvest tea leaves under high temperature (38 °C), low temperature (4 °C), and shading spreadings by using ultrahigh-performance liquid chromatography. The differentially expressed proteins (DEPs), CsFd-GOGAT and CsNADH-GOGAT, which are involved in theanine biosynthesis pathway, were identified from the corresponding proteome data. The protein-protein interactions of CsFd-GOGAT and CsNADH-GOGAT, CsTS1, or CsNiR were verified by yeast two-hybrid technology. The expression profiles of 17 genes in theanine metabolism, including CsFd-GOGAT and CsNADH-GOGAT, were analyzed by quantitative real-time polymerase chain reaction. The correlations between the dynamic changes of theanine content and expression profiles of related genes and DEPs were analyzed. This study preliminarily proved the importance of CsGOGAT in dynamic changes of theanine content in postharvest tea leaves during spreading.
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| http://dx.doi.org/10.1021/acs.jafc.7b04552 | DOI Listing |
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