Lysophosphatidic acid acyltransferases (LPAATs) are essential for the acylation of lysophosphatidic acid (LPA) and the synthesis of phosphatidic acid (PA), a key intermediate in the synthesis of membrane phospholipids and storage lipids. Here, a putative lysophosphatidic acid acyltransferase gene, designated , was isolated from seed unsaturated fatty acid (UFA)-rich . The complete cDNA contained a 1116-bp open reading frame (ORF), encoding a 42.9 kDa protein with 371 amino acid residues. Bioinformatic analysis indicates that PrLPAAT4 is a plasma membrane protein belonging to acyl-CoA:1-acylglycerol-sn-3-phosphate acyltranferases (AGPAT) family. PrLPAAT4 shared high sequence similarity with its homologs from , , , and . In Arabidopsis, overexpression of resulted in a significant increase in the content of oleic acid (OA) and total fatty acids (FAs) in seeds. , , and , involved in TAG assembly, were upregulated in -overexpressing lines. These results indicated that PrLPAAT4 functions may be as a positive regulator in seed FA biosynthesis.
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| http://dx.doi.org/10.3390/molecules22101694 | DOI Listing |
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