Aim: Clinical significance of plant homeodomain finger 2 (PHF2) expressions is explored in acute lymphoblastic leukemia (ALL) patients.
Methods: mRNA level was examined by qPCR. The retroviral gene expression, shRNA knockdown and chromatin-immunoprecipitation are used to observe IKAROS regulation on PHF2 transcription.
Results: PHF2 expression is significantly reduced in subsets of ALL patients, and PHF2 expression correlates with leukemia cell proliferation and an elevation of several poor prognostic markers in B-cell ALL. IKAROS directly promotes PHF2 expression and patients with IKAROS deletion have significantly lower PHF2 expression. Casein kinase II (CK2) inhibitor significantly promotes PHF2 expression in an IKAROS-dependent manner, and casein kinase II inhibitor treatment also results in an increase of PHF2 expression and enrichment of IKAROS and H3K4me3 at PHF2 promoter in primary cells.
Conclusion: Our results demonstrate that the IKAROS promotes PHF2 expression, and suggest that PHF2 expression works with the IKAROS gene deletion to drive oncogenesis of ALL.
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| http://dx.doi.org/10.2217/epi-2017-0092 | DOI Listing |
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