The two-dimensional difference gel electrophoresis method is a valuable approach for proteomics. The method, using cyanine fluorescent dyes, allows the co-migration of multiple protein samples in the same gel and their simultaneous detection, thus reducing experimental and analytical time. 2D-DIGE, compared to traditional post-staining 2D-PAGE protocols (e.g., colloidal Coomassie or silver nitrate), provides faster and more reliable gel matching, limiting the impact of gel to gel variation, and allows also a good dynamic range for quantitative comparisons. By the use of internal standards, it is possible to normalize for experimental variations in spot intensities and gel patterns. Here we describe the experimental steps we follow in our routine 2D-DIGE procedure that we then apply to multiple biological questions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-7231-9_17 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of a unique crosslinked glycosaminoglycan for soft tissue repair: Treatment of interstitial cystitis/bladder pain syndrome.
PLoS One
January 2025
Glycologix, Inc., 100 Cummings Center, Beverly, Massachusetts, United States of America.
Chemical modification of naturally derived glycosaminoglycans (GAGs) expands their potential utility for applications in soft tissue repair and regenerative medicine. Here we report the preparation of a novel crosslinked chondroitin sulfate (~200 to 2000 kilodaltons) that is both soluble in aqueous solution and microfilterable. We refer to these materials as "SuperGAGs.
View Article and Find Full Text PDFRespiratory extracellular vesicle isolation optimization through proteomic profiling of equine samples and identification of candidates for cell-of-origin studies.
PLoS One
January 2025
Curriculum in Toxicology & Environmental Medicine, UNC Chapel Hill, Chapel Hill, North Carolina, United States of America.
Growing evidence supports the importance of extracellular vesicle (EV) as mediators of communication in pathological processes, including those underlying respiratory disease. However, establishing methods for isolating and characterizing EVs remains challenging, particularly for respiratory samples. This study set out to address this challenge by comparing different EV isolation methods and evaluating their impacts on EV yield, markers of purity, and proteomic signatures, utilizing equine/horse bronchoalveolar lavage samples.
View Article and Find Full Text PDFLaboratory and in-home evaluations of consumer- and professional-grade cockroach baits.
J Econ Entomol
January 2025
Department of Entomology, University of Kentucky, Lexington, KY, USA.
German cockroaches (Blattella germanica (L.)) are a persistent pest in affordable housing and studies indicate that residents implement control on their own to deal with cockroaches within their homes. While many do-it-yourself (DIY) control options have proven ineffective, baits are widely considered to be a viable DIY solution for residents who do not have access to professional pest control services.
View Article and Find Full Text PDFBlastocystis ST1: Protein Profile and Specific Serum Immunoglobulin in Irritable Bowel Syndrome (IBS) Patients.
Acta Parasitol
January 2025
Department of Animal Biology, Faculty of Science, Damascus University, Damascus, Syria.
Purpose: Blastocystis sp. is a common enteric human parasite, which recently has been linked to gastrointestinal disorders i.e.
View Article and Find Full Text PDFOxidative and nitrative DNA damage induced by industrial chemicals in relation to carcinogenesis.
J Occup Health
January 2025
Department of Environmental Health, University of Fukui School of Medical Science, Eiheiji, Fukui, Japan.
Objectives: Many chemicals have been used for industrial purposes, and some of them are carcinogenic to humans. However, their molecular mechanisms have not been well understood. Reactive oxygen species are generated from industrial chemicals and contribute to carcinogenesis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!