AI Article Synopsis

  • Touch spray mass spectrometry is an innovative technique that can quickly analyze tissue samples during surgery to identify brain cancer and assess tumor margins in real-time.
  • A study on 29 brain tumor specimens demonstrated that this method effectively extracts critical molecular information using a medical swab combined with specific solvents and high voltage.
  • It allows for the assessment of tumor cell presence and mutation status without needing extensive sample preparation, making it a practical tool for surgeons.

Article Abstract

Touch spray mass spectrometry using medical swabs is an ambient ionization technique (ionization of unprocessed sample in the open air) that has potential intraoperative application in quickly identifying the disease state of tissue and in better characterizing the resection margin. To explore this potential, we studied 29 human brain tumor specimens and obtained evidence that this technique can provide diagnostic molecular information that is relevant to brain cancer. Touch spray using medical swabs involves the physical sampling of tissue using a medical swab on a spatial scale of a few mm with subsequent ionization occurring directly from the swab tip upon addition of solvent and application of a high voltage. Using a tertiary mixture of acetonitrile, N,N-dimethylformamide, and ethanol, membrane-derived phospholipids and oncometabolites are extracted from the tissue, incorporated into the sprayed microdroplets, vacuumed into the mass spectrometer, and characterized in the resulting mass spectra. The tumor cell load was assessed from the complex phospholipid pattern in the mass spectra and also separately by measurement of N-acetylaspartate. Mutation status of the isocitrate dehydrogenase gene was determined via detection of the oncometabolite 2-hydroxyglutarate. The lack of sample pretreatment makes touch spray mass spectrometry using medical swabs a feasible intraoperative strategy for rapid surgical assessment.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653971PMC
http://dx.doi.org/10.1039/c7an01334eDOI Listing

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