AI Article Synopsis
- The study aimed to assess how effective electron paramagnetic resonance (EPR) oximetry is for monitoring oxygen levels in wounds treated with a specific peptide (hCAP-18/LL37) in diabetic mice.
- Diabetic mice of different ages (7 and 12 weeks old) with varying levels of blood vessel disease were used, and EPR oximetry monitored the oxygenation in the treated wounds, showing notable differences between the age groups.
- Results indicated that younger treated mice exhibited improved wound reoxygenation, less hypoxia, and faster healing compared to untreated mice, suggesting EPR oximetry could be a useful measure for evaluating wound treatment effectiveness.
Article Abstract
Purpose: To investigate the value of electron paramagnetic resonance oximetry to follow oxygenation in wounds treated by a plasmid-encoding host defense peptide hCAP-18/LL37.
Methods: Flaps were created on diabetic mice (7- or 12-week-old db/db mice) presenting different levels of microangiopathy. The hCAP-18/LL37-encoding plasmids were administered in wounds by electroporation. Low-frequency electron paramagnetic resonance oximetry using lithium phthalocyanine as the oxygen sensor was used to monitor wound oxygenation in flaps during the healing process. Flaps were analyzed by immunohistochemistry to assess hypoxia and cell proliferation. Kinetics of closure was also assessed in excisional skin wounds.
Results: A reoxygenation of the flap was observed during the healing process in the 7-week-old db/db treated mice, but not in the untreated mice and the 12-week-old mice. Histological studies demonstrated less hypoxic regions and higher proportion of proliferating cells in hCAP-18/LL37-treated flaps in the 7-week-old db/db treated mice compared with untreated mice. Consistently, the kinetics of excisional wound closure was improved by hCAP-18/LL37 treatment in the 7-week-old db/db but not in the 12-week-old mice.
Conclusions: Oxygenation measured by electron paramagnetic resonance oximetry is a promising biomarker of response to treatments designed to modulate wound oxygenation. Magn Reson Med 79:3267-3273, 2018. © 2017 International Society for Magnetic Resonance in Medicine.
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| http://dx.doi.org/10.1002/mrm.26956 | DOI Listing |
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