Artemia diapause has been extensively studied in embryonic biology for a long time. It has been demonstrated that hydrogen peroxide (HO) can increase the hatching rate in the development and diapause termination of Artemia cysts. This study used an untargeted H NMR-based metabolomic approach to explore the physiological regulation of HO in initiating the development and terminating the diapause of Artemia cysts. This experiment was divided into two parts. In the first part, we analyzed three groups with or without HO as control-0h, control-5h and HO (180μM)-5h; in the second part, after 7-d incubation, the non-hatching cysts were treated with different HO concentrations as low as 180μM and as high 1800μM. The results showed that arginine and proline metabolism were up-regulated after 5h, and HO up-regulated valine, leucine and isoleucine biosynthesis in the development of cysts. In the second part, low HO (180μM) showed alanine, aspartate and glutamate metabolism, but high HO (1800μM) also up-regulated arginine and proline metabolism, as in the control group without HO stimulus. These results suggest that enough HO can catalyze cell transcription and translation in Artemia cysts, and it improves the cell growth rate, thus allowing embryo cells to grow again.
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