AI Article Synopsis
- Toxoplasma gondii, a eukaryotic parasite from the Apicomplexa phylum, causes toxoplasmosis in warm-blooded animals and humans, with rhoptry neck proteins (RONs) being crucial for invasion and potential vaccine targets.
- The study analyzed sequence variations in the TgRON10 gene from 10 different isolates in Lanzhou province, utilizing PCR amplification and sequence analysis to investigate genetic differences and construct phylogenetic relationships.
- Results indicated low sequence variation in the TgRON10 gene, with 7 exons and 6 introns identified; thus, while it isn't ideal for distinguishing between isolates, the TgRON10 gene shows promise as a vaccine candidate against toxoplasmosis.
Article Abstract
Background: as a eukaryotic parasite of the phylum Apicomplexa, can infect almost all the warm-blooded animals and humans, causing toxoplasmosis. Rhoptry neck proteins (RONs) play a key role in the invasion process of and are potential vaccine candidate molecules against toxoplasmosis.
Methods: The present study examined sequence variation in the rhoptry neck protein 10 (TgRON10) gene among 10 isolates from different hosts and geographical locations from Lanzhou province during 2014, and compared with the corresponding sequences of strains ME49 and VEG obtained from the ToxoDB database, using polymerase chain reaction (PCR) amplification, sequence analysis, and phylogenetic reconstruction by Bayesian inference (BI) and maximum parsimony (MP).
Results: Analysis of all the 12 TgRON10 genomic and cDNA sequences revealed 7 exons and 6 introns in the TgRON10 gDNA. The complete genomic sequence of the TgRON10 gene ranged from 4759 bp to 4763 bp, and sequence variation was 0-0.6% among the 12 isolates, indicating a low sequence variation in TgRON10 gene. Phylogenetic analysis of TgRON10 sequences showed that the cluster of the 12 isolates was not completely consistent with their respective genotypes.
Conclusion: TgRON10 gene is not a suitable genetic marker for the differentiation of isolates from different hosts and geographical locations, but may represent a potential vaccine candidate against toxoplasmosis, worth further studies.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5623912 | PMC |
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