AI Article Synopsis
- Cell adhesion to the extracellular matrix (ECM) through focal adhesions (FAs) is essential for cell functions like survival and movement.
- Autophagy plays a role in both the attachment and detachment of cells, affecting how FAs behave and are regulated.
- The study indicates that when autophagy is deficient, cells have more internalized collagen and altered paxillin distribution, leading to enhanced cell adhesion and impaired signaling related to focal adhesion kinase.
Article Abstract
Adhesion of cells to the extracellular matrix (ECM) via focal adhesions (FAs) is crucial for cell survival, migration, and differentiation. Although the regulation of FAs, including by integrins and the ECM, is important to cell behavior, how FAs are regulated is not well known. Autophagy is induced by both cell adhesion and cell detachment. Here, we showed that autophagosomes are located close to internalized collagen and paxillin, which is a well-known marker of FAs. Autophagy-deficient cells showed increased levels of internalized collagen compared with control cells. Moreover, paxillin exhibited a more peripheral distribution and the area of paxillin was increased, and adhesion-induced focal adhesion kinase signaling was impaired and adhesion was enhanced, in autophagy-deficient cells. These results suggest that autophagy suppressed cell adhesion by regulating internalized ECM and FAs.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703610 | PMC |
| http://dx.doi.org/10.1242/bio.027458 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bacterial-host adhesion dominated by collagen subtypes remodelled by osmotic pressure.
NPJ Biofilms Microbiomes
November 2024
Department of Mechanics and Engineering Science, College of Engineering, Peking University, Beijing, China.
Environmental osmolarity plays a crucial role in regulating the functions and behaviors of both host cells and pathogens. However, it remains unclear whether and how environmental osmotic stimuli modulate bacterial‒host interfacial adhesion. Using single-cell force spectroscopy, we revealed that the interfacial adhesion force depended nonlinearly on the osmotic prestimulation of host cells but not bacteria.
View Article and Find Full Text PDFExosomes in nanomedicine: a promising cell-free therapeutic intervention in burn wounds.
Stem Cell Res Ther
October 2024
Division of Animal Biotechnology, Faculty of Veterinary Sciences & Animal Husbandry, SKUAST, Srinagar, Kashmir, 190006, India.
Burn injuries are serious injuries that have a big impact on a person's health and can even cause death. Incurring severe burns can incite an immune response and inflammation within the body, alongside metabolic changes. It is of utmost importance to grasp the fact that the effects of the burn injury extend beyond the body, affecting the mind and overall well-being.
View Article and Find Full Text PDFMSC-extracellular vesicle microRNAs target host cell-entry receptors in COVID-19: in silico modeling for in vivo validation.
Stem Cell Res Ther
September 2024
Department of Medical Histology and Cell Biology Faculty of Medicine, Benha University, Benha, Egypt.
Background: Coronavirus disease 2019 (COVID-19) has created a global pandemic with significant morbidity and mortality. SARS-CoV-2 primarily infects the lungs and is associated with various organ complications. Therapeutic approaches to combat COVID-19, including convalescent plasma and vaccination, have been developed.
View Article and Find Full Text PDFCollagen/PCL electrospun fibers loaded with polyphenols: Curcumin and resveratrol comparison.
Int J Biol Macromol
November 2024
Department of Drug Sciences, University of Pavia, Pavia, Italy.
Article Synopsis
- Curcumin (Cur) and resveratrol (Rsv) have potential uses in both anti-tumor and wound healing treatments, and their effects on blood vessel formation (angiogenesis) can vary based on the intended application.
- This study compared electrospun fibers made from collagen and polycaprolactone (PCL) loaded with Cur and Rsv, finding that Rsv-loaded fibers were larger and had better wettability.
- Results showed both types of fibers were biocompatible, leading to an increase in fibroblast cells, and both exhibited anti-angiogenic properties, indicating a stronger suitability for anti-cancer applications rather than wound healing.
WRN Nuclease-Mediated EcDNA Clearance Enhances Antitumor Therapy in Conjunction with Trehalose Dimycolate/Mesoporous Silica Nanoparticles.
Adv Sci (Weinh)
October 2024
State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, 300350, China.
Article Synopsis
- Current research mainly focuses on cancer-associated fibroblasts and their complex role in tumor dynamics, but there's a lack of studies on whether tumor cells themselves can undergo fibrotic transformation.
- This study uses mesoporous silica nanoparticles loaded with Trehalose dimycolate (TDM) to induce fibrosis in tumor cells, which results in collagen accumulation and reduced tumor growth and spread.
- To counteract resistance from extrachromosomal DNA (ecDNA), the researchers combine TDM with WRN exonuclease to create a new nanoparticle (TMW) that effectively promotes tumor cell transformation and inhibits cancer progression, indicating TMW's potential as a treatment strategy.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!