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Investigation of Salmonella Enteritidis outbreaks in South Africa using multi-locus variable-number tandem-repeats analysis, 2013-2015. | LitMetric

AI Article Synopsis

  • Salmonella enterica serovar Enteritidis has emerged as a major pathogen in South Africa, highlighting the necessity for enhanced molecular surveillance methods, particularly the multi-locus variable-number tandem-repeats analysis (MLVA) technique for outbreak investigation.* -
  • The study analyzed 39 Salmonella Enteritidis isolates from seven outbreaks between 2013 and 2015, identifying three distinct MLVA profiles, with profile 28 representing 77% of the cases, indicating a strong genetic relationship among the isolates.* -
  • The findings demonstrate that MLVA is an effective tool for investigating Salmonella Enteritidis outbreaks, underscoring the importance of a One Health approach to manage the spread of zoonotic diseases.*

Article Abstract

Background: Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) has become a significant pathogen in South Africa, and the need for improved molecular surveillance of this pathogen has become important. Over the years, multi-locus variable-number tandem-repeats analysis (MLVA) has become a valuable molecular subtyping technique for Salmonella, particularly for highly homogenic serotypes such as Salmonella Enteritidis. This study describes the use of MLVA in the molecular epidemiological investigation of outbreak isolates in South Africa.

Methods: Between the years 2013 and 2015, the Centre for Enteric Diseases (CED) received 39 Salmonella Enteritidis isolates from seven foodborne illness outbreaks, which occurred in six provinces. MLVA was performed on all isolates.

Results: Three MLVA profiles (MLVA profiles 21, 22 and 28) were identified among the 39 isolates. MLVA profile 28 accounted for 77% (30/39) of the isolates. Isolates from a single outbreak were grouped into a single MLVA profile. A minimum spanning tree (MST) created from the MLVA data showed a close relationship between MLVA profiles 21, 22 and 28, with a single VNTR locus difference between them.

Conclusions: MLVA has proven to be a reliable method for the molecular epidemiological investigation of Salmonella Enteritidis outbreaks in South Africa. These foodborne outbreaks emphasize the importance of the One Health approach as an essential component for combating the spread of zoonotic pathogens such as Salmonella Enteritidis.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5625639PMC
http://dx.doi.org/10.1186/s12879-017-2751-8DOI Listing

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