Haemophilus influenzae protein F (PF) is an important virulence factor interacting with laminin, an extracellular matrix protein ubiquitously expressed in the respiratory tract. Here we defined PF orthologs in Pseudomonas aeruginosa, Moraxella catarrhalis, and Staphylococcus aureus, bacteria that occasionally colonize and infect the human airways. Despite low sequence homology (48.2%-77.3% similarity), all orthologs (Paf, AfeA, and MntC) interacted with laminin. Interestingly, all proteins bound at the heparin-binding sites of laminin, including the globular domains, and also attached to laminin expressed on respiratory epithelial cells. Laminin is thus a highly important target for PF orthologs of the bacterial species examined.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/infdis/jix467 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Predictive value of plasma heparin-binding protein combined with albumin for 28-day mortality in patients with sepsis].
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
December 2024
Department of Emergency Medicine, People's Hospital of Shenzhen Baoan District (the Second Affiliated Hospital of Shenzhen University), Shenzhen 518101, Guangdong, China. Corresponding author: Dou Qingli, Email:
Objective: To evaluate the predictive value of plasma heparin-binding protein (HBP) combined with albumin (Alb) for predicting 28-day mortality in patients with sepsis.
Methods: The clinical data of patients with sepsis admitted to the emergency intensive care unit (EICU) of the People's Hospital of Shenzhen Baoan District from March 2020 to March 2024 were retrospectively analyzed. The study began at the time of the first diagnosis of sepsis upon EICU admission and ended upon patient death or at 28 days.
The concept of natural genome reconstruction.Part 1. Basic provisions of the "natural genome reconstruction" concept. Changing the genome of hematopoietic stem cells using several natural cellular mechanisms that are inherent in the hematopoietic cell and determine its biological status as "the source of the body's reparative potential".
Vavilovskii Zhurnal Genet Selektsii
November 2024
Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
We present a series of articles proving the existence of a previously unknown mechanism of interaction between hematopoietic stem cells and extracellular double-stranded DNA (and, in particular, double-stranded DNA of the peripheral bloodstream), which explains the possibility of emergence and fixation of genetic information contained in double-stranded DNA of extracellular origin in hematopoietic stem cells. The concept of the possibility of stochastic or targeted changes in the genome of hematopoietic stem cells is formulated based on the discovery of new, previously unknown biological properties of poorly differentiated hematopoietic precursors. The main provisions of the concept are as follows.
View Article and Find Full Text PDFElucidating the Mechanism of Recognition and Binding of Heparin to Amyloid Fibrils of Serum Amyloid A.
Biochemistry
January 2025
Department of Chemistry, Boston University, Boston, Massachusetts 02215, United States.
Amyloid diseases feature pathologic deposition of normally soluble proteins and peptides as insoluble fibrils in vital organs. Amyloid fibrils co-deposit with various nonfibrillar components including heparan sulfate (HS), a glycosaminoglycan that promotes amyloid formation in vitro for many unrelated proteins. HS-amyloid interactions have been proposed as a therapeutic target for inflammation-linked amyloidosis wherein N-terminal fragments of serum amyloid A (SAA) protein deposit in the kidney and liver.
View Article and Find Full Text PDFSynthesis, Biological Evaluation and Reversal of Sulfonated Di- and Triblock Copolymers as Novel Parenteral Anticoagulants.
Adv Healthc Mater
December 2024
Department of Pharmacodynamics, Medical University of Bialystok, Mickiewicza 2C St., Bialystok, 15-089, Poland.
Article Synopsis
- FDA-approved anticoagulants can increase bleeding risks, including serious intracranial hemorrhages, but the specific effectiveness and safety of sulfonate polymers as alternatives have not been thoroughly studied.
- Researchers synthesized and evaluated various sulfonated copolymers, finding that PSSS-based copolymers showed stronger anticoagulant effects compared to PAMPS-based ones, with the effectiveness influenced by sulfonate concentration and molecular weight.
- The PEG-PSSS copolymer demonstrated significant anticoagulant activity in animal models and can be reversed by Heparin Binding Copolymer (HBC), targeting specific factors in the blood coagulation process, indicating potential for use in medical applications related to blood contact.
Structural and biochemical investigation into stable FGF2 mutants with novel mutation sites and hydrophobic replacements for surface-exposed cysteines.
PLoS One
September 2024
Marine Biotechnology & Bioresource Research Department, Korea Institute of Ocean Science and Technology, Busan, Republic of Korea.
Fibroblast growth factor 2 (FGF2) is an attractive biomaterial for pharmaceuticals and functional cosmetics. To improve the thermo-stability of FGF2, we designed two mutants harboring four-point mutations: FGF2-M1 (D28E/C78L/C96I/S137P) and FGF2-M2 (D28E/C78I/C96I/S137P) through bioinformatics, molecular thermodynamics, and molecular modeling. The D28E mutation reduced fragmentation of the FGF2 wild type during preparation, and the substitution of a whale-specific amino acid, S137P, enhanced the thermal stability of FGF2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!