AI Article Synopsis
- The study investigates improving ethyl lactate (EL) production using enzymatic esterification, comparing various organic solvents for their effectiveness.
- Chloroform and hexane were found to be the best solvents at low acid concentrations, yielding up to 88% and 75% EL, respectively, with higher LogP values correlating to better yields.
- While commercial enzymes like Novozym 435 were the most efficient, non-commercial biocatalysts showed promise for hydrolysis, providing a cost-effective option despite lower overall esterification performance.
Article Abstract
This study explores the potential for enhancing the production of ethyl lactate (EL), a green solvent, through enzymatic esterification. Different solvents were compared as organic media for conversion of lactate and ethanol into EL, catalyzed by Novozym 435. Chloroform and hexane were the most effective in low acid concentrations (0.01-0.1M) exhibiting maximum EL yields of 88% and 75% respectively. The yield of EL improved as the solvent's LogP increased up to a value of 2. Non-commercial immobilized biocatalysts consisting heterologous Rhizopous oryzae (rROL) and Candida rugosa (CRL) lipases immobilized on hydrophobic supports were compared to commercial biocatalysts clarifying that Novozym 435 and Lipozyme RM IM could be efficiently applied. Operational stability tests were conducted using Novozym 435, which retained higher activity in chloroform as compared to hexane. Although non-commercial biocatalysts were not competitive in esterification, they exhibited significant activity towards hydrolysis constituting a valuable alternative to higher-cost options.
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| http://dx.doi.org/10.1016/j.biortech.2017.09.130 | DOI Listing |
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