AI Article Synopsis
- This study introduces a new imaging technique that combines structured illumination microscopy (SIM) and multifocus microscopy (MFM) to enhance super-resolution imaging.
- The method aims to solve the problem of slow volumetric acquisition speeds in biological imaging, making it easier to capture detailed images quickly.
- The researchers show that this improved technique allows for simultaneous wide-field 3D imaging while significantly speeding up the volumetric acquisition process.
Article Abstract
We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in super-resolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging capability in the SIM acquisition sequence, improving volumetric acquisition speed by an order of magnitude. Imaging performance is demonstrated on biological specimens.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611928 | PMC |
| http://dx.doi.org/10.1364/BOE.8.004135 | DOI Listing |
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