causes hairy root growth on a large number of plant species. It does so by transferring specific DNA fragments (T-DNA) from its root-inducing plasmid (pRi) into plant cells. Expression of T-DNA genes leads to abnormal root growth and production of specific metabolites (opines) which are taken up by the bacterium and used for its growth. Recent work has shown that several , and species contain T-DNA genes from in their genomes. Plants carrying such T-DNAs (called cellular T-DNA or cT-DNA) can be considered as natural transformants. In the genus, seven different T-DNAs are found originating from different strains, and in the section no <4 successive insertion events took place. In several cases cT-DNA genes were found to be expressed. In some cultivars the opine synthesis gene TB-' is expressed in the roots. These cultivars were found to produce opines. Here we review what is known about natural transformants, develop a theoretical framework to analyze this unusual phenomenon, and provide some outlines for further research.
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| http://dx.doi.org/10.3389/fpls.2017.01600 | DOI Listing |
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