A single-step, homogeneous and sensitive LRET assay is presented for the detection of miRNAs. The amplification-free assay provides a unique combination of high specificity with dual-recognition approach of different hybridization and ligation steps and preventing background auto-fluorescence in biological samples using upconversion nanoparticles (UCNPs) as signal-producing nanoprobes. The assay probe is composed of signal-producing unit (a pair of homogeneous upconversion luminescence resonance energy transfer (UC-LRET)-based oligonucleotides) and recognition unit (two adaptor oligonucleotides). In the presence of target miRNAs, the probe and target miRNAs leads to the formation of stable double-strands and semi-stable adaptor-miRNAs complexes with an adaptor nick. Ligation of the nick using ligase cause the formation of stable double-strands, resulting in UCNPs-to-dye UC-LRET for detection of the miRNAs with near-infrared radiation (980nm). Sensitive detection of miRNA-21 at concentrations of 200pM to 1.4nM and detection limits of 0.095nM with good precision of 3.9% (RSD) for seven repeated measurements of 500pM miRNAs demonstrate the feasibility of both high throughput and point-of-care clinical diagnostics. The homogeneous UC-LRET assay without any washing can be extended to the application in other important types of nucleic acid analysis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bios.2017.09.039 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Article Synopsis
- Recent studies spotlight MBenes, a new type of two-dimensional material, but their production is still challenging.
- Microcrystalline MoAlB was synthesized through a one-step gas-solid reaction at 450 °C, which can serve as a precursor for creating MoB MBenes.
- This innovative method offers a more efficient, cost-effective way to produce layered materials, leading to uniform microcrystals and having promising implications for applications in catalysis and energy storage.
Selective recovery of Co(II), Mn(II), Cu(II), and Ni(II) by multiple step batch treatments with nanocellulose products.
Environ Sci Pollut Res Int
December 2024
Cellulose, Paper and Advanced Water Treatments Research Group, Department of Chemical Engineering, Complutense University of Madrid, Avda. Complutense S/N, Madrid, Spain.
The recovery of Co(II), Mn(II), Ni(II), and Cu(II) from black mass e-waste solutions through cellulose nanofibers (CNFs) and nanocrystals (CNCs) was investigated. These materials were synthetized by TEMPO-oxidation followed by high-pressure homogenization, and acid hydrolysis, respectively. The NC characterization included the measurement of consistency, cationic demand, carboxylic content, dissolved amorphous cellulose, and transmittance at λ = 600 nm.
View Article and Find Full Text PDFFabrication of Ultrafine L1-FePt-Based Magnetic Patterns Enabled by Single-Step Nanoimprint-Assisted Block Copolymer Self-Assembly.
Nano Lett
November 2024
Department of Applied Biology and Chemical Technology and Research Institute for Smart Energy, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China.
The bit islands in magnetic patterns play a crucial role in advancing magnetic recording density, but the trade-off issues between miniaturization and scalable production are still challenging. Here we present a two-in-one technique of nanoimprint lithography (NIL)-assisted self-assembly using a specially engineered FePt-containing block copolymer (BCP), offering a simple one-step fabrication for L1-FePt bit-patterned media with high throughput. This method combines top-down NIL with bottom-up self-assembly to precisely control the ultrafine magnetic bits in the nanoscale patterns.
View Article and Find Full Text PDFElectrospun chitosan//ethylcellulose-vitamin E//ethylcellulose-curcumin tri-chamber eccentric Janus nanofibers for a joint antibacterial and antioxidant performance.
Int J Biol Macromol
November 2024
Department of Periodontology, Shanghai Stomatological Hospital & School of Stomatology, Fudan University, Shanghai 200001, China; Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai 200001, China. Electronic address:
Multifunctional materials with both antibacterial and antioxidant properties are highly desired in many scientific applications. The combination of polysaccharide and multi-chamber nanostructures offers a novel perspective for developing antibacterial and antioxidant nanomaterials. In this study, a new kind of tri-chamber eccentric Janus nanostructures (TEJNs) was fabricated through a single-step and straight forward tri-fluid side-by-side electrospinning.
View Article and Find Full Text PDFSingle-Step Extrusion Process for Formulation Development of Self-Emulsifying Granules for Oral Delivery of a BCS Class IV Drug.
Mol Pharm
December 2024
Pharmaceutical Engineering and 3D Printing (PharmE3D) Lab, Department of Pharmaceutics and Drug Delivery, School of Pharmacy, University of Mississippi, University, Mississippi 38677, United States.
Article Synopsis
- This study focuses on improving the solubility and permeability of a BCS Class IV drug by developing self-emulsifying solid lipid matrices as a new form of drug delivery system.
- Self-emulsifying drug delivery systems (SEDDS) are helpful for poorly soluble drugs but face manufacturing challenges like low drug loading and stability issues.
- The research explores using a single-step extrusion process to create solid self-emulsifying granules with higher drug loading, which demonstrated significantly improved solubility and permeability, enhancing the potential for effective drug delivery.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!