Increasing complexity and diversity of polymersomes and their compartments is a key issue for mimicking cellular functions and protocells. Thus, new challenges arise in terms of achieving tunable membrane permeability and combining it with control over the membrane diffusion process, and thus enabling a localized and dynamic control of functionality and docking possibilities within or on the surface of polymeric compartments. This study reports the concept of polymersomes with pH-tunable membrane permeability for controlling sequential docking and undocking processes of small molecules and nanometer-sized protein mimics selectively on the inside and outside of the polymersome membrane as a further step toward the design of intelligent multifunctional compartments for use in synthetic biology and as protocells. Host-guest interactions between adamantane and β-cyclodextrin as well as noncovalent interactions between poly(ethylene glycol) tails and β-cyclodextrin are used to achieve selective and dynamic functionalization of the inner and outer spheres of the polymersome membrane.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/marc.201700486 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparison of localization and release of multivesicular bodies and secretory granules in islet cells: Dysregulation during type-2 diabetes.
J Extracell Biol
November 2024
Cell Metabolism Lab (GA-08), Department of Developmental Biology and Genetics (DBG) Indian Institute of Science (IISc) Bengaluru India.
Multivesicular bodies (MVBs) are vesicles of endosomal origin containing intraluminal vesicles, which upon fusion with plasma membrane, secrete exosomes. They play a significant role in the physiology and pathology of type-2 diabetes (T2D) due to disrupted intercellular communication. The role of MVBs and their influence on insulin secretory granules (ISGs) of β-cells or their characterization is yet to be uncovered.
View Article and Find Full Text PDFClinical value of capsule endoscopy, CT enterography and enteroscopy in the diagnosis of suspected small bowel bleeding.
Surg Endosc
November 2024
Jiangxi Provincial Key Laboratory of Digestive Diseases, Department of Gastroenterology, Jiangxi Clinical Research Center for Gastroenterology, Digestive Disease Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, 17 Yongwaizheng Street, Nanchang, 330006, Jiangxi, China.
Article Synopsis
- - The study evaluated the effectiveness of three diagnostic techniques—capsule endoscopy, CT enterography, and enteroscopy—for detecting small bowel bleeding, highlighting the need for more research in this area.
- - Findings showed enteroscopy had a significantly higher diagnostic rate compared to both capsule endoscopy and CT enterography, indicating it is the most dependable method for diagnosing suspected small bowel bleeding.
- - The results also revealed that successful completion of the docking inspection during enteroscopy greatly improves diagnostic rates, while there was no significant difference in outcomes between one-day and non-one-day enteroscopy groups.
Modeling of Chemomechanical Coupling of Cytoplasmic Dynein Motors.
J Phys Chem B
October 2024
Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.
Cytoplasmic dynein homodimer is a motor protein that can step processively on microtubules (MTs) toward the minus end by hydrolyzing ATP molecules. Some dynein motors show a complicated stepping behavior with variable step sizes and having both hand-overhand and inchworm steps, while some mammalian dynein motors show simplistic stepping behavior with a constant step size and having only hand-overhand steps. Here, a model for the chemomechanical coupling of the dynein is presented, based on which an analytical theory is given on the dynamics of the motor.
View Article and Find Full Text PDFPrevalence of tail damage and ear lesions in docked and undocked pigs during trials to find alternatives to tail docking on Spanish commercial farms.
Vet Rec
October 2024
Animal Welfare Program, Institute of Agrifood Research and Technology, Monells, Spain.
Background: Tail docking is widely used to control tail biting in pigs, but it is a painful and stressful procedure. The impact of non-docking trials on tail damage (TD) and ear lesions (EL) from weaning to slaughter was assessed on four commercial farms.
Methods: A total of 898 docked pigs (396 DP) or undocked pigs (502 UP) were assessed for tail damage and ear lesions at the beginning and end of the weaner stage, during fattening and at the abattoir during slaughter.
Mispacking of the F87 sidechain drives aggregation-promoting conformational fluctuations in the subunit interfaces of the transthyretin tetramer.
Protein Sci
September 2024
Department of Integrative Structural and Computational Biology and Skaggs Institute of Chemical Biology, The Scripps Research Institute, La Jolla, California, USA.
Aberrant formation and deposition of human transthyretin (TTR) aggregates causes transthyretin amyloidosis. To initialize aggregation, transthyretin tetramers must first dissociate into monomers that partially unfold to promote entry into the aggregation pathway. The native TTR tetramer (T) is stabilized by docking of the F87 sidechain into an interfacial cavity enclosed by several hydrophobic residues including A120.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!