Green Nanoparticles Engineering on Root-knot Nematode Infecting Eggplants and Their Effect on Plant DNA Modification.

Background: Root-knot nematodes are known to cause significant damage to eggplants. New approaches by green silver nanoparticles (GSN) are used to control plant-parasitic nematode to avoid chemical nematicide hazards.

Objectives: Analyses of the incorporation of different concentrations of nanoparticles on two different algae ( and ) were carried out. Furethermore, the effect of GSN on the eggplant DNA profile was studied using RAPD and EST molecular markers.

Materials And Methods: Green Silver Nanoparticles (GSN) have been synthesized and characterized using the algal extract solution prepared from two algal genera. Nematicidal effect of the GSN was evaluated in greenhouse on eggplants ( Login). Genomic DNA was extracted for use in molecular analysis. Both RAPD and EST molecular markers were used to study the GSN effect on eggplant DNA modification.

Results: GSN (17 mg.mL) obtained from was more effective in reducing second-stage juveniles (J2s) of (69.44%) population in soil. All treatments improved eggplants growth parameters. Change in DNA profile using of both RAPD and EST markers was noted.

Conclusions: GSN (12.75 mg.100 mL) were effective on controlling the root-knot nematode (both and algae), similar to chemical control in eggplants. GSN did not cause any phototoxicity in eggplants under treatment.