Background: Identification and quantification of mycotoxins produced by species are important in controlling fungal diseases.
Objectives: Potential of zearalenone, butenolide and production was investigated in five and five isolates at molecular level.
Materials And Methods: Presence of and genes, associated with production of zearalenone, butenolide and , respectively, were confirmed by PCR. In addition, expression levels of them together with housekeeping gene () were detected by real time PCR.
Results: and transcripts were determined in all isolates, while PKS10 specific primers failed to amplify any product, indicative of no expression. ΔΔCTCT of was ranged between 1.79E-03-3.97E-03 and for was between 0.25E-03 and 6.02E-03. The highest expressions were 3.86E-03 in F9 and 3.97E-03 in F16. Maximum expressions were calculated as 6.02E-03 and 3.81E-03 in 2F and F2, respectively.
Conclusions: We revealed that ten isolates produced zearalenone and butenolide under culture conditions. However, fusarin C was not generated by them in these conditions.
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