Mycobacteria encode five type VII secretion system (T7SS) or ESX for nutrient acquisition and virulence. Mycosins are membrane-anchored components of ESX with serine protease activity but an unidentified substrate range. Establishing the substrate specificity of individual mycosins will help to elucidate individual ESX functions. Mycosin-1 and -3 orthologues from two environmental mycobacterial species, and , have been heterologously produced, but mycosins from () remain to be studied. Here we describe the successful production of mycosin-3 as a first step in investigating its structure and function.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600315 | PMC |
| http://dx.doi.org/10.1016/j.bbrep.2016.02.005 | DOI Listing |
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