AI Article Synopsis
- The transcriptional regulator Wor1 plays a key role in the GUT transition, enhancing fitness in the mouse gastrointestinal tract in response to environmental changes.
- Researchers created strains with controlled Wor1 expression using a tetracycline promoter, which retained GUT cell characteristics but struggled in early colonization stages.
- These modified cells displayed unique colonization patterns and metabolic changes, suggesting they could be better at adhering to the mouse mucosa and thus enhance their long-term colonization capabilities.
Article Abstract
The transcriptional regulator Wor1 has been shown to induce the GUT transition, an environmentally triggered process that increases the fitness of in the mouse gastrointestinal tract. We have developed strains where the expression of this gene is driven from the strong and tightly regulated tetracycline promoter. These cells retain the main characteristics reported for GUT cells albeit they show defects in the initial stages of colonization. They also show a differential colonization along the gastrointestinal tract compared to isogenic strains, which is probably caused by their susceptibility to bile salts. We also show that overexpressing cells have an altered metabolic activity, as revealed by a different susceptibility to inhibitors of respiration, and an enhanced adhesion to the mouse mucosa. We propose that this may contribute to their long-term favored ability to colonize the gastrointestinal tract.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600957 | PMC |
| http://dx.doi.org/10.3389/fcimb.2017.00389 | DOI Listing |
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