AI Article Synopsis
- A devastating bacterial pathogen affects freshwater-reared salmonids globally, and while serological diversity among strains has been noted, the specific molecular factors are still unclear.
- By analyzing complete genomes and applying a serotyping method, researchers identified important molecular determinants across 34 strains, leading to the creation of a multiplex PCR-based serotyping scheme.
- This new scheme, tested on 244 bacterial isolates, showed a strong link between serotype and fish species, proving useful for disease monitoring, enhancing disease resistance in salmonids, and aiding vaccine development.
Article Abstract
is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes. This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of serogroups. PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5601056 | PMC |
| http://dx.doi.org/10.3389/fmicb.2017.01752 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!