AI Article Synopsis
Article Abstract
This paper presents a systematic investigation into monomer development during mixed culture Polyhydroxyalkanoates (PHA) accumulation involving concurrent active biomass growth and polymer storage. A series of mixed culture PHA accumulation experiments, using several different substrate-feeding strategies, was carried out. The feedstock comprised volatile fatty acids, which were applied as single carbon sources, as mixtures, or in series, using a fed-batch feed-on-demand controlled bioprocess. A dynamic trend in active biomass growth as well as polymer composition was observed. The observations were consistent over replicate accumulations. Metabolic flux analysis (MFA) was used to investigate metabolic activity through time. It was concluded that carbon flux, and consequently copolymer composition, could be linked with how reducing equivalents are generated.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5590436 | PMC |
| http://dx.doi.org/10.3390/bioengineering4010020 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Specialized pericyte subtypes in the pulmonary capillaries.
EMBO J
January 2025
Division of Pulmonary Medicine, Boston Children's Hospital, Boston, MA, 02115, USA.
Pericytes are essential for capillary stability and homeostasis, with impaired pericyte function linked to diseases like pulmonary arterial hypertension. Investigating pericyte biology has been challenging due to the lack of specific markers, making it difficult to distinguish pericytes from other stromal cells. Using bioinformatic analysis and RNAscope, we identified Higd1b as a unique gene marker for pericytes and subsequently generated a knock-in mouse line, Higd1b-CreERT2, that accurately labels pericytes in the lung and heart.
View Article and Find Full Text PDFAge-related changes in bioelectrical impedance analysis-derived phase angle (PhA) and the association between PhA and sarcopenic obesity in Chinese adults.
Medicine (Baltimore)
January 2025
Department of Clinical Nutrition, Tongde Hospital of Zhejiang Province, Hangzhou, China.
There are limited studies on the phase angle (PhA) and sarcopenic obesity (SO) in the Chinese population. This study aimed to establish 50 kHz-PhA reference data for SO population, and to evaluate the correlation between 50 kHz-PhA and SO. A total of 10,312 participants including 5415 men and 4897 women were enrolled in this study, and their resistance and reactance at 50 kHz, and body composition parameters were measured a segmental multifrequency bioelectrical impedance analysis device (InBody 720).
View Article and Find Full Text PDFA genome-scale metabolic model for the denitrifying bacterium Thauera sp. MZ1T accurately predicts degradation of pollutants and production of polymers.
PLoS Comput Biol
January 2025
Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, California, United States of America.
The denitrifying bacterium Thauera sp. MZ1T, a common member of microbial communities in wastewater treatment facilities, can produce different compounds from a range of carbon (C) and nitrogen (N) sources under aerobic and anaerobic conditions. In these different conditions, Thauera modifies its metabolism to produce different compounds that influence the microbial community.
View Article and Find Full Text PDFAssessing the efficiency and potential for internally reusing nitrogen-containing effluent in the PHA accumulation stage under low C/N conditions in a mixed-culture process.
Bioresour Technol
December 2024
School of Water Conservancy and Transportation, Zhengzhou University, Zhengzhou 450001, China. Electronic address:
Article Synopsis
- Polyhydroxyalkanoates (PHAs) are biodegradable plastics that can be produced through a mixed culture-based process, but ammonia nitrogen can hinder this production.
- This study explores ways to efficiently reuse ammonia nitrogen to enhance PHA synthesis and reduce waste.
- Results showed a significant increase in PHA production when using specific substrate and process conditions, while also effectively recycling ammonia without negatively affecting the mixed culture's properties.
Lignocellulosic biomass as promising substrate for polyhydroxyalkanoate production: Advances and perspectives.
Biotechnol Adv
December 2024
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China. Electronic address:
The depletion of fossil resources, coupled with global warming and adverse environmental impact of traditional petroleum-based plastics, have necessitated the discovery of renewable resources and innovative biodegradable materials. Lignocellulosic biomass (LB) emerges as a highly promising, sustainable and eco-friendly approach for accumulating polyhydroxyalkanoate (PHA), as it completely bypasses the problem of "competition for food". This sustainable and economically efficient feedstock has the potential to lower PHA production costs and facilitate its competitive commercialization, and support the principles of circular bioeconomy.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!