[Analysis on genetic diversity and genetic relationship of medicinal species in Coptis by SCoT].

Zhongguo Zhong Yao Za Zhi

Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.

Published: February 2017

AI Article Synopsis

  • The study used sequence-related amplified polymorphism (SCoT) to assess genetic diversity and relationships among four medicinal Coptis species.
  • A total of 434 genetic bands were produced, with a high level of genetic diversity observed between species but lower diversity within species.
  • The clustering analysis indicated that most genetic variations occur among species, confirming that SCoT markers are effective in revealing genetic relationships in these medicinal plants.

Article Abstract

The genetic diversity and genetic relationship among four medicinal species of Coptis were detected by the approach of sequence-related amplified polymorphism (SCoT). The associated genetic parameters were calculated by POPGENE1.31. The systematic diagram of genetic relationship were clustered by TREECONW. The results showed that a total of 434 bands were produced by using 28 primers, of which 430 were polymorphic loci. There was a high level of genetic diversity among species (PPB=99.1%,Na=1.990 6,Ne=1.329 3,H=0.212 2,I=0.337 8). However, genetic diversity was lower within species, the average of genetic parameters wasPPB=16.8%,Na=1.168 2, Ne=1.073 0,H=0.043 7,I=0.067 7. The Nei's genetic differentiation coefficient was 0.794 0, that indicated that most of the genetic variation existed among species. By clustering analysis, different individuals gathered in the same group. The results confirmed that SCoT marker can be used as one of the effective methods to reveal the genetic diversity and relationship among medicinal species of Coptis.

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http://dx.doi.org/10.19540/j.cnki.cjcmm.20170103.029DOI Listing

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