AI Article Synopsis
- Progesterone is crucial for the maintenance of gestation and protects against luteolysis in mice, and this study investigates its relationship with Slit/Robo signaling in the corpus luteum (CL).
- Real-time PCR was employed to assess the impact of progesterone on Slit2/Robo1 expression both in cultured luteal cells (in vitro) and in mouse models (in vivo).
- Results indicated that progesterone treatment led to a significant decrease in Slit2/Robo1 mRNA and protein levels, suggesting that progesterone may help maintain CL function by downregulating the Slit/Robo signaling pathway, thereby preventing luteolysis.
Article Abstract
Background: Progesterone produced by the corpus luteum (CL) is essential for preparation, implantation and maintenance of gestation. Furthermore, progesterone plays a protective role against luteolysis in rodents. It has been reported that Slit/Robo family members expressed in the CL and involved in prostaglandin F (PGF) induced luteolysis. However, the interactions between progesterone and Slits/Robos in CL are not clear. This study was designed to examine whether or not luteolysis is regulated by the interaction of progesterone and Slits/Robos in mouse CL.
Methods: In the current study, we used Real-time PCR to identify the effect of progesterone on Slit2/Robo1 expression in cultured luteal cells in vitro, and the exogenous progesterone injection on mouse luteolysis and Slit/Robo expression in vivo was studied via Real-time PCR and Western bolt.
Results: Our in vitro experiment revealed that 1μM progesterone significantly decreased Slit2/Robo1 mRNA levels at 6h, 12h and 24h. Our in vivo experiment showed that the mRNA and protein levels of Slit2 and Robo1 decreased significantly 7days after progesterone supplement.
Conclusion: These findings indicate that progesterone maintains CL function and resists luteolysis possibly through down-regulating Slit/Robo signaling pathway in the CL.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.acthis.2017.09.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Role of resistin in the porcine uterus: effects on endometrial steroidogenesis.
Reprod Fertil Dev
January 2025
Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Context The adipose tissue produces adipokines - hormones essential to many biological functions, including reproduction. Aims We hypothesised that resistin, one of the adipokines, is present in the blood plasma, uterine luminal flushings (ULF) and uterus of pigs during the oestrous cycle and early pregnancy, and that resistin influences uterine steroidogenesis. Methods This study aimed to determine the expression of resistin in the porcine endometrium and myometrium during the cycle and pregnancy by quantitative real-time polymerase chain reaction and western blot (WB).
View Article and Find Full Text PDFDeveloping Topics.
Alzheimers Dement
December 2024
Brighton & Sussex Medical School, Brighton, United Kingdom.
Background: Reported effects of Hormone Replacement Therapy (HRT) on late-life neurodegenerative disease are inconsistent. Variability in the timing and formulation of HRT, plus whether an individual carries an Apolipoprotein (APOE) e4 genetic risk variant for Alzheimer's Disease (AD), likely contribute to conflicting results. Additionally, whilst many studies have focused exclusively on the effects of exogenous oestrogen, the inclusion of testosterone in HRT appears protective against AD pathology, specifically in APOE e4 carriers.
View Article and Find Full Text PDFDeveloping Topics.
Alzheimers Dement
December 2024
Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Background: Evidence suggests that a history of reduced estrogen exposure associates with greater Alzheimer's disease (AD) risk. However, the association of sex hormone levels with AD biomarkers has not been studied. We examined plasma levels of sex hormones in males and females with autosomal dominant AD due to the E280A Presenilin-1 mutation and age-matched non-carriers, and their relationship to AD brain pathology.
View Article and Find Full Text PDFAlzheimer's Imaging Consortium.
Alzheimers Dement
December 2024
Texas A&M University, College Station, TX, USA.
Background: Older females, particularly susceptible to Alzheimer's disease (AD), may be affected by hormonal fluctuation during life. We aim to investigate the relationship between changes in brain volume and sex steroid hormones over time. We hypothesize that levels of sex hormones (17ß-estradiol, progesterone, and testosterone) relate to changes in brain volume, especially in the hippocampus (HPC) and cerebellum (CB).
View Article and Find Full Text PDFOccult breast cancer in an older woman: A case report.
Exp Ther Med
February 2025
Department of Breast Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
Occult breast cancer (OBC) is a relatively rare clinical condition that can complicate differential diagnosis efforts and delay the administration of specific treatments. The individualized therapy of patients with OBC should be performed based on their clinical symptoms, imaging findings and pathological diagnosis. The present case study describes a 51-year-old woman with a painless left axillary tumor.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!