AI Article Synopsis
- Bacterial infections in seeds significantly impact agricultural yield, but traditional detection methods like biological and molecular tests are costly and slow.
- This study introduces Raman hyperspectral imaging as a quick, accurate, and non-destructive way to identify bacteria-infected watermelon seeds.
- By analyzing spectral data, two optimal Raman peaks (1076.8 cm and 437 cm) were identified, showing the technique's promise as a viable alternative to conventional testing methods.
Article Abstract
The bacterial infection of seeds is one of the most important quality factors affecting yield. Conventional detection methods for bacteria-infected seeds, such as biological, serological, and molecular tests, are not feasible since they require expensive equipment, and furthermore, the testing processes are also time-consuming. In this study, we use the Raman hyperspectral imaging technique to distinguish bacteria-infected seeds from healthy seeds as a rapid, accurate, and non-destructive detection tool. We utilize Raman hyperspectral imaging data in the spectral range of 400-1800 cm to determine the optimal band-ratio for the discrimination of watermelon seeds infected by the bacteria using ANOVA. Two bands at 1076.8 cm and 437 cm are selected as the optimal Raman peaks for the detection of bacteria-infected seeds. The results demonstrate that the Raman hyperspectral imaging technique has a good potential for the detection of bacteria-infected watermelon seeds and that it could form a suitable alternative to conventional methods.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677267 | PMC |
| http://dx.doi.org/10.3390/s17102188 | DOI Listing |
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