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Domain-switch analysis of PeNHX3 from Populus euphratica reveals the critical role of the transmembrane domain 11 in Na and Li transport. | LitMetric

Domain-switch analysis of PeNHX3 from Populus euphratica reveals the critical role of the transmembrane domain 11 in Na and Li transport.

J Plant Physiol

MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, 73000, China. Electronic address:

Published: December 2017

AI Article Synopsis

Article Abstract

Populus euphratica, the well-known tree halophyte, tolerates the stress of high levels of salt. We previously showed that the transmembrane domain 11 (TM11) of PeNHX3, a Na,K/H antiporter from P. euphratica, was crucial for Na and Li transport in a yeast growth assay. Here, we examined the role of TM11 in catalyzing Na and Li transport in transgenic Arabidopsis. We found that PeNHX3 localized to the tonoplasts in Arabidopsis. Overexpression of PeNHX3 in Arabidopsis improved seedling growth and enhanced salt tolerance and Li detoxification. However, overexpression of PeNHX3 did not improve Arabidopsis growth at KCl concentrations higher than 0.1mM, suggesting a low K transport activity for PeNHX3 in plants. We performed in planta domain-switch analysis by replacing the C-terminal domain of AtNHX1 with a C-terminal segment of PeNHX3 containing the TM11 domain. We demonstrated that TM11 was critical for the Na and Li transport activities by PeNHX3. Taken together, PeNHX3 plays an important role in salt tolerance and Li detoxification in plants. TM11 controls the Na and Li transport activities of PeNHX3 in Arabidopsis.

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Source
http://dx.doi.org/10.1016/j.jplph.2017.09.003DOI Listing

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