Wohlfahrtiimonas populi sp. nov., isolated from symptomatic bark of a Populus × euramericana canker.

A Gram-stain-negative, facultatively anaerobic, motile, bacterial strain, 34C10-3-10, was isolated from symptomatic bark tissue of a Populus ×euramericana canker. The isolate could grow between 10 and 37 °C, at pH 5 to 11, and in 0-3 % (w/v) NaCl. The strain was oxidase and catalase positive. The ubiquinone of strain 34C10-3-10 was Q-8. The polar lipid profile of strain 34C10-3-10 included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipid, phosphatidylmonomethylethanolamine, phosphatidylserine; the major fatty acids were C18 : 1ω7c, C14 : 0 and C16 : 1ω7c/C16 : 1ω6c. The average nucleotide identity (ANI) values between strain 34C10-3-10 and the type strains of reference species Wohlfahrtiimonas chitiniclastica S5 and Wohlfahrtiimonas larvae KBL006 were lower than the proposed species boundary cut-off for ANI. The DNA G+C content was 37.1 mol%. Based on phenotypic and genotypic characteristics, strain 34C10-3-10 represents a novel species of genus Wohlfahrtiimonas; the name Wohlfahrtiimonas populi sp. nov. is proposed. The type strain is 34C10-3-10 (=CFCC 12747=KCTC 52796).