Single-cell microfluidic devices are poised to substantially impact the hematology field by providing a high-throughput and rapid device to analyze disease-mediated biophysical cellular changes in the clinical setting in order to diagnose patients and monitor disease prognosis. In this Feature, we cover recent advances of single-cell microfluidic devices for studying and diagnosing hematological dysfunctions and the clinical impact made possible by these advances.
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| http://dx.doi.org/10.1021/acs.analchem.7b01013 | DOI Listing |
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Bulk methods to fractionate organelles lack the resolution to capture single-cell heterogeneity. While microfluidic approaches attempt to fractionate organelles at the cellular level, they fail to map each organelle back to its cell of origin-crucial for multiomics applications. To address this, we developed VacTrap, a high-throughput microfluidic device for isolating and spatially indexing single nuclei from mammalian cells.
View Article and Find Full Text PDFHighly Efficient Dual-Probe Strategy toward Single-Cell Metabolite Analysis.
Anal Chem
January 2025
School of Public Health, Nantong Key Laboratory of Public Health and Medical Analysis, Nantong University, Nantong 226019, P. R. China.
Article Synopsis
- As cancer progresses, detached cells spread via the bloodstream, leading to complex metabolic changes that aid their survival and growth, yet the process of metastasis is still not fully understood.
- A new microfluidic platform utilizing a dual-probe strategy allows for the detection of metabolic changes at single-cell resolution, enabling researchers to analyze both intracellular and extracellular environments in detail.
- Findings indicate a positive relationship between increased intracellular NAD(P)H and higher levels of matrix metalloproteinases (MMPs), suggesting that these insights could aid in developing zinc-based treatments targeting cancer metastasis.
Tumor cell-based liquid biopsy using high-throughput microfluidic enrichment of entire leukapheresis product.
Nat Commun
January 2025
Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA.
Circulating Tumor Cells (CTCs) in blood encompass DNA, RNA, and protein biomarkers, but clinical utility is limited by their rarity. To enable tumor epitope-agnostic interrogation of large blood volumes, we developed a high-throughput microfluidic device, depleting hematopoietic cells through high-flow channels and force-amplifying magnetic lenses. Here, we apply this technology to analyze patient-derived leukapheresis products, interrogating a mean blood volume of 5.
View Article and Find Full Text PDFATLAS-seq: a microfluidic single-cell TCR screen for antigen-reactive TCRs.
Nat Commun
January 2025
Raymond G. Perelman Center for Cellular and Molecular Therapeutics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Discovering antigen-reactive T cell receptors (TCRs) is central to developing effective engineered T cell immunotherapies. However, the conventional technologies for isolating antigen-reactive TCRs (i.e.
View Article and Find Full Text PDFStepwise isolation of diverse metabolic cell populations using sorting by interfacial tension (SIFT).
Lab Chip
January 2025
Department of Chemistry and Biochemistry, Santa Clara University, Santa Clara, CA, 95053, USA.
We present here a passive and label-free droplet microfluidic platform to sort cells stepwise by lactate and proton secretion from glycolysis. A technology developed in our lab, Sorting by Interfacial Tension (SIFT), sorts droplets containing single cells into two populations based on pH by using interfacial tension. Cellular glycolysis lowers the pH of droplets through proton secretion, enabling passive selection based on interfacial tension and hence single-cell glycolysis.
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