Fluorescence Microscopy of Nanochannel-Confined DNA.

Methods Mol Biol

NanoLund and Department of Physics, Lund University, PO Box 118, 22100, Lund, Sweden.

Published: May 2018

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.

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Source
http://dx.doi.org/10.1007/978-1-4939-7271-5_10DOI Listing

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