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Techniques for detecting protein-protein interactions in living cells: principles, limitations, and recent progress.
Sci China Life Sci
May 2019
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, 100083, China.
Detecting protein-protein interactions (PPIs) provides fundamental information for understanding biochemical processes such as the transduction of signals from one cellular location to another; however, traditional biochemical techniques cannot provide sufficient spatio-temporal information to elucidate these molecular interactions in living cells. Over the past decade, several new techniques have enabled the identification and characterization of PPIs. In this review, we summarize three main techniques for detecting PPIs in vivo, focusing on their basic principles and applications in biological studies.
View Article and Find Full Text PDFCAPPI: A Cytoskeleton-Based Localization Assay Reports Protein-Protein Interaction in Living Cells by Fluorescence Microscopy.
Mol Plant
November 2017
Department of Plant Biology, University of California, Davis, CA 95616, USA. Electronic address:
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