The 9th International Countercurrent Chromatography Conference (CCC 2016) was held at Dominican University near Chicago, IL (USA), from August 1st-3rd, 2016. The biennial CCC 20XX conferences provide an opportunity for countercurrent chromatography and centrifugal partition chromatography (CCC/CPC) manufactures, marketers, theorists, and research scientists to gather together socially, learn from each other, and advance countercurrent separation technology. A synopsis of the conference proceedings as well as a series of short reviews of the special edition articles is included in this document. Many productive discussions and collegial conversation at CCC 2016 attested to the liveliness, connectivity, and productivity of the global countercurrent research community and bodes well for the success of the 10th conference at the University of Braunschweig, Germany on August 1-3, 2018.
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http://dx.doi.org/10.1016/j.chroma.2017.08.077 | DOI Listing |
J Chromatogr Sci
January 2025
Characteristic Biology Resources Research Center, Northwest Institute of Plateau Biology, Chinese Academy of Science, No. 23, Xinning Road, Chengxi District, Xining, Qinghai 810001, P. R. China.
Separation of polar compounds especially with similar polarities is challenging. In the present study, three polar compounds with similar polarities, including gentiopicroside, sweroside and mangiferin, have been successfully separated from Swertia mussotii by a combination of two counter-current chromatography modes. With the selected solvent system of ethyl acetate/n-butanol/water (8/2/10, v/v), a continuous injection mode was firstly employed.
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December 2024
Department of Food Chemistry (170B), Institute of Food Chemistry, University of Hohenheim, Garbenstraβe 28, D-70599 Stuttgart, Germany.
The exploration of natural antifungal substances from algal origins is significant due to the increasing resistance of pathogens to conventional antifungal agents and the growing consumer demand for natural products. This manuscript represents the inaugural investigation into the antifungal attributes of bioactive compounds extracted from via supercritical carbon dioxide (scCO) extraction utilizing contemporary countercurrent chromatography (CCC). In aligning with the prospective utilization of this extract within the agricultural sector, this study also serves as the preliminary report demonstrating the capability of scCO extract to enhance the activity of plant resistance enzymes.
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December 2024
Amur Branch of Botanical Garden-Institute, Far Eastern Branch of Russian Academy of Sciences, Blagoveshchensk 675000, Russia.
An antiviral effect of extracts prepared from aerial parts of nine species and from leaves of two species of the genus L. was investigated for potential antiviral activity toward influenza A (H1N1) virus. The toxicity of dry extracts was analyzed, and the most selective extract was identified in vitro.
View Article and Find Full Text PDFJ Chromatogr A
January 2025
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China; Key Laboratory of Traditional Chinese Medicine Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China. Electronic address:
Countercurrent chromatography (CCC) is an efficient technique for purifying bioactive natural compounds, but selecting the solvent system can be a time-consuming and crucial process for successful separation. This paper discussed the HPLC-assisted mathematical prediction method for the n-hexane/alcohol solvents/water (HAWat) and ethyl acetate/n-butanol/water (EBuWat) systems and designed an intelligent online selection system to simplify the separation process. First, the applicable rage of HAWat and EBuWat solvent systems were quantified by the methanol concentration at the column inlet when template molecules peak in a HPLC analysis (B%).
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December 2024
School of Pharmacy and Pharmaceutical Sciences, Panoz Institute, and Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, D02 PN40 Dublin, Ireland.
is a lesser-known plant species belonging to the genus that grows in Kazakhstan. The aim of this study was to characterize the composition of the ethanolic, water, and hydroethanolic extracts from the aerial parts of by HPLC-ESI-QTOF-MS/MS to isolate the major compound isoquercitrin by HSCCC (High-Speed Counter-Current Chromatography) and to determine the cytotoxicity and anti-inflammatory potential of the extracts produced with this plant. Fingerprinting of the analyzed extracts showed the presence of a multitude of metabolites comprising polyphenols, organic acids, and coumarins, and only trace quantities of cardiac glycosides in the analyzed samples.
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