AI Article Synopsis
- Draft whole-genome sequences were created for two bacterial strains known to produce bulgecins and NRPS-derived monobactam β-lactam antibiotics, with ATCC 31363 identified as Paraburkholderia acidophila.
- ATCC 31433 is confirmed as a distinct producer of bulgecins, showing genetic similarities to Paraburkholderia acidophila.
- Analysis using RAST and MASH identified unique gene regions and 48 natural-product gene clusters, highlighting 10 genes likely responsible for bulgecin A biosynthesis.
Article Abstract
We have produced draft whole-genome sequences for two bacterial strains reported to produce the bulgecins as well as NRPS-derived monobactam β-lactam antibiotics. We propose classification of ATCC 31363 as Paraburkholderia acidophila. We further reaffirm that ATCC 31433 (Burkholderia ubonensis subsp. mesacidophila) is a taxonomically distinct producer of bulgecins with notable gene regions shared with Paraburkholderia acidophila. We use RAST multiple-gene comparison and MASH distancing with published genomes to order the draft contigs and identify unique gene regions for characterization. Forty-eight natural-product gene clusters are presented from PATRIC (RASTtk) and antiSMASH annotations. We present evidence that the 10 genes that follow the sulfazecin and isosulfazecin pathways in both species are likely involved in bulgecin A biosynthesis.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653948 | PMC |
| http://dx.doi.org/10.1021/acschembio.7b00687 | DOI Listing |
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