AI Article Synopsis
- Wnt signaling is crucial for developing airway epithelial cells and plays a key role in the redifferentiation of ciliated cells in vitro.
- Researchers identified phases of cell proliferation and differentiation while examining changes in cell types and ciliation.
- Enhancing β-catenin signaling reduces ciliated cells and causes squamous changes, while inhibiting this signaling with Dkk1 increases ciliated cell numbers.
Article Abstract
Wnt signaling is essential for the differentiation of airway epithelial cells during development. Here, we examined the role of Wnt signaling during redifferentiation of ciliated airway epithelial cells in vitro at the air liquid interface as a model of airway epithelial repair. Phases of proliferation and differentiation were defined. Markers of squamous metaplasia and epithelial ciliation were followed while enhancing β-catenin signaling by blocking glycogen synthase kinase 3β with SB216763 and shRNA as well as inhibiting canonical WNT signaling with apical application of Dickkopf 1 (Dkk1). Our findings indicate that enhanced β-catenin signaling decreases the number of ciliated cells and causes squamous changes in the epithelium, whereas treatment with DDk1 leads to an increased number of ciliated cells.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5683904 | PMC |
| http://dx.doi.org/10.1002/1873-3468.12851 | DOI Listing |
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