Background: The main pathogen of neonatal and post weaning diarrhea and edema disease (ED) is and pathotypes involved are enterotoxigenic, enteropathogenic, and shiga toxigenic (ETEC, EPEC, and STEC, respectively). Those diseases cause economic loss in pig production.
Aim: The aim of this work was to evaluate the presence of strains expressing virulence markers genes and the antibiotic susceptibility profiles of from clinical cases of post weaning diarrhea and ED in farms in the central area of Argentina.
Materials And Methods: Intensive pig farms from the central region of Argentina were sampled. Intestinal mucosa swabs from pigs with diarrhea were taken, seeded on MacConkey agar plates, biochemically typified and tested by polymerase chain reaction (PCR). Antibiograms were made by disk-diffusion method.
Results: A total of 54 strains from clinical cases studied showed PCR findings: 88.88% (48/54) expressed at least one gene coding for a virulence factor. Colonization factors found were: 39.58% of strains had , 33.33% were and 31.25% adhesin involved in diffuse adherence-I; 29.17%, 25%, and 2.1% expressed , , and , respectively. 25% were and 16.67% were positive. Only 2.1% were . The most active antibiotics against most strains were gentamicin and ceftiofur, but resistance profiles against many antibiotics were found.
Conclusion: High circulation of pathogens strains of among pigs with diarrhea with an extended antibiotic resistance profile.
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| http://dx.doi.org/10.14202/vetworld.2017.939-945 | DOI Listing |
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