Aim: The present work aimed to develop lateral flow immunochromatographic strip (ICS) test for detection of Enteritidis (SE) specific antibodies in chicken sera.
Materials And Methods: A rapid lateral flow immunochromatographic test (LFIT) has been developed, in which SE Group D antigen labeled with the gold chloride molecules laid on the conjugate pad. protein A was used as capture antibody at the test line (T) of a nitrocellulose (NC) membrane and anti-SE antigen-specific rabbit antibodies were used as capture antibody at the control line (C) of the NC strip in the lateral flow layout device.
Results: Using the developed LFIT, the minimal amount of SE-specific antibodies that can be detected in chicken serum sample was 1427 enzyme-linked immunosorbent assay (ELISA) unit/100 µl that was equal to 0.1 µg (Ab)/100 µl sample. 100 suspected serum samples collected from a poultry flock were tested with the prepared SE-LFIT kits and the locally prepared stained antigen, and the results were compared with those obtained from examination of these samples with Group D antibody ELISA kit as the gold standard test. The sensitivity, specificity, and accuracy of the prepared SE-LFIT antigen kits were 94.4%, 90%, and 94%, respectively, while those obtained with stained antigen were 88.8%, 90%, and 89%, respectively.
Conclusion: The developed test is a simple field rapid test of high sensitivity, specificity, and accuracy that can improve and facilitates rapid field surveillance of salmonellosis among chickens.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591467 | PMC |
| http://dx.doi.org/10.14202/vetworld.2017.848-853 | DOI Listing |
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