AI Article Synopsis
- The study investigates the anti-Acanthamoeba activity of essential oils derived from the peels of Tunisian Citrus sinensis, particularly focusing on the impact of viroid infections on this activity.
- Three samples were analyzed: one from a healthy plant, one infected with Citrus exocortis viroid (CEVd), and another infected with hot stunt cachexia viroid (HSVd).
- Results indicated that essential oils have significant anti-Acanthamoeba potential with limonene as the main component, and viroid infections positively influenced their effectiveness.
Article Abstract
In order to promote a local Tunisian product, this study was designed to examine, for the first time, the anti-Acanthamoeba activity (Acanthamoeba castellanii Neff) of the essential oils of Tunisian Citrus sinensis peels (Maltese half-blood) and the effect of viroid plant infection on this activity. To do so, three samples of peels' essential oils were studied: from a healthy plant (Control), a plant inoculated with Citrus exocortis viroid (CEVd) and one inoculated with hot stunt cachexia viroid (HSVd). The samples were extracted by hydrodistillation from dried peels and characterized by GC-MS. Limonene was the major component with a percentage ranging from 90.76 to 93.34% for (CEVd) sample and (Control), respectively. Anti-Acanthamoeba activity of the tested oils was determined by the Alamar Blue assay. Primary results showed a strong potential anti-Acanthamoeba activity with an IC ranging from 36.6 to 54.58 μg/ml for (HSVd) and (CEVd) samples, respectively. In terms of the effect of viroid infection, a strong positive correlation was observed between different chemical classes and anti-Acanthamoeba activity.
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| http://dx.doi.org/10.1016/j.exppara.2017.09.006 | DOI Listing |
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