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High-Density SNP Genotyping Array for Hexaploid Wheat and Its Relatives. | LitMetric

High-Density SNP Genotyping Array for Hexaploid Wheat and Its Relatives.

Methods Mol Biol

School of Biological Sciences, University of Bristol, Bristol Life Sciences Building, 24 Tyndall Ave, Bristol, BS8 1TH, UK.

Published: May 2018

AI Article Synopsis

  • Wheat breeding faces challenges due to low genetic diversity, which limits potential yield increases, prompting the need for diversity reintroduction using ancestors and wild relatives.
  • Molecular markers help track the introgressed genetic material through generations, and sequence capture targeted resequencing was performed on various wheat varieties and relatives to identify useful markers.
  • The identified SNPs led to the creation of the Axiom® Wheat HD array, which was validated using a selection of elite breeding materials and wild relatives, with detailed procedures outlined in the text.

Article Abstract

A lack of genetic diversity between wheat breeding lines has been recognized as a significant block to future yield increases. Wheat breeding and prebreeding strategies are increasingly using material from wheat ancestors or wild relatives to reintroduce diversity. Where molecular markers are polymorphic between the host and introgressed material, they may be used to track the size and location of the introgressed material through generations of backcrossing. To generate markers for this purpose, sequence capture targeted resequencing was carried out for a range of wheat varieties, wheat relatives, and wheat progenitors. From these sequences, putative SNPs were identified and used to generate the Axiom® Wheat HD array. A selection of varieties representing a selection of elite wheat breeding material, progenitor species, and wild relatives were used to validate the array. The procedures used are described here in detail.

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Source
http://dx.doi.org/10.1007/978-1-4939-7337-8_19DOI Listing

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