Effective partial nitrification of ammonia in a fluidized bed bioreactor.

A lab-scale fluidized bed bioreactor with high-density polyethylene as biofilm carrier media was operated to study partial nitrification (PN) performance with high ammonia concentrations. The system was run at nitrogen loading rates (NLRs) from 1.2 to 4.8 kg N/(m d) with empty bed contact time of 2.0 and 2.7 h and four different influent ammonia concentrations of 100, 200, 300 and 400 mg/L. Dissolved oxygen concentration and temperature were maintained around 1.3 mg/L and 35°C, respectively. Stable PN was successfully achieved during the whole period with low effluent NO-N concentration at less than 15 mg/L, due to effective suppression of nitrite-oxidizing bacteria activity at high concentrations of free ammonia (5.3-27.3 mg N/L) and low alkalinity-to-ammonia ratio. At the NLR of 3.6 kg N/(m d), NH-N conversion and NO-N accumulation ratios were 57.8% and 53.9%, respectively, which could be further used in the anaerobic ammonium oxidation process (ANAMMOX) as the effluent NO-N/NH-N ratio was 1.27.