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| http://dx.doi.org/10.1128/JCM.01437-17 | DOI Listing |
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Analysis of Time-to-Positivity Data in Tuberculosis Treatment Studies: Identifying a New Limit of Quantification.
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UCSF Center for Tuberculosis, University of California, San Francisco, San Francisco, California, USA; Division of Pulmonary and Critical Care Medicine, University of California, San Francisco, San Francisco, California, USA.
Background: The BACTEC Mycobacteria Growth Indicator Tube (MGIT) machine is the standard globally for detecting viable mycobacteria in patients' sputum. Samples are observed for no longer than 42 days, at which point the sample is declared 'negative' for tuberculosis (TB). This time to detection of bacterial growth, referred to as time-to-positivity (TTP), is increasingly of interest, not solely as a diagnostic tool but also as a continuous biomarker wherein change in TTP can be used for comparing the bactericidal activity of different TB treatments.
View Article and Find Full Text PDFComparative evaluation of BacT/ALERT VIRTUO and BACTEC FX400 blood culture systems for the detection of bloodstream infections.
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Department of Laboratory Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, China.
Bloodstream infections (BSI) pose significant threats to patient health, necessitating timely and accurate diagnostics to reduce mortality and morbidity. This study aimed to evaluate the clinical performance of the BacT/ALERT VIRTUO blood culture system with FANPlus bottles compared to the BACTEC FX400 system in detecting bloodstream pathogens. A total of 1,772 blood specimens were collected from various hospital wards.
View Article and Find Full Text PDFA simplified pyrazinamidase test for pyrazinamide antimicrobial susceptibility testing.
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Tuberculosis Research Center, Centers for Disease Control, Ministry of Health and Welfare, Taipei, Taiwan.
Unlabelled: Pyrazinamide (PZA) is an important first-line drug for tuberculosis (TB) treatment by eradicating the persisting complex (MTBC). Due to cost and technical challenges, end TB strategies are hampered by the lack of a simple and reliable culture-based PZA antimicrobial susceptibility testing (AST) for routine use. We initially developed a simplified chromogenic pyrazinamidase (PZase) test in the TB reference laboratory using a training set MTBC isolates with various drug-resistant profiles, and validated its performance using consecutive BACTEC MGIT 960 (MGIT)-culture-positive culture in 10 clinical laboratories.
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View Article and Find Full Text PDFMycobacterium tuberculosis complex sample processing by mechanical lysis, an essential step for reliable whole genome sequencing.
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Department Bacterial Surveillance and Response, Center for Infectious Disease Control, Diagnostics and Laboratory Surveillance, National Institute for Public Health and the Environment (RIVM), 3721 MA Bilthoven, the Netherlands. Electronic address:
Article Synopsis
- Whole genome sequencing (WGS) of Mycobacterium tuberculosis complex (MTBC) is greatly affected by the effectiveness of DNA extraction protocols, particularly the lysis step.
- In a study comparing four different DNA extraction methods, a bead beating protocol (protocol B) significantly increased DNA yield by 60-fold and improved sequencing success rates.
- Incorporating mechanical lysis not only enhances extraction from primary cultures but also enables earlier WGS from enriched cultures, ultimately reducing turnaround time while maintaining high-quality DNA needed for analysis.
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