The synthesis of 2'-O-methyl-5-hydroxymethylcytidine (hm Cm), 5-hydroxymethylcytidine (hm C) and 5-formylcytidine (f C) phosphoramidite monomers has been developed. Optimisation of mild post-synthetic deprotection conditions enabled the synthesis of RNA containing all four naturally occurring cytosine modifications (hm Cm, hm C, f C plus 5-methylcytosine). Given the considerable interest in RNA modifications and epitranscriptomics, the availability of synthetic monomers and RNAs containing these modifications will be valuable for elucidating their biological function(s).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5779611 | PMC |
| http://dx.doi.org/10.1002/cbic.201700492 | DOI Listing |
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