AI Article Synopsis
- The PII protein family is a highly conserved group of signal transduction proteins important for nitrogen and carbon metabolism, mainly studied in Gram-negative bacteria.
- There is limited understanding of PII proteins from Gram-positive bacteria, particularly from Actinobacteria significant in medicine and biotechnology.
- This study details successful methods for expressing and purifying untagged PII proteins from Actinobacteria, confirming their correct folding and paving the way for further biochemical research on their functions.
Article Abstract
The PII protein family constitutes one of the most conserved and well distributed family of signal transduction proteins in nature. These proteins play key roles in nitrogen and carbon metabolism. PII function has been well documented in Gram-negative bacteria. However, there are very few reports describing the properties and function of PII derived from Gram-positive bacteria. Here we present the heterologous expression and efficient purification protocols for untagged PII from three Actinobacteria of medical and biotechnological interest namely: and Circular dichroism and gel filtration analysis supported that the purified proteins are correctly folded. The purification protocol described here will facilitate biochemical studies and help to uncover the biochemical functions of PII proteins in Actinobacteria.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5579400 | PMC |
| http://dx.doi.org/10.17179/excli2017-394 | DOI Listing |
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