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Application of laboratory and digital techniques for visual enhancement during the ultrastructural assessment of cilia. | LitMetric

Application of laboratory and digital techniques for visual enhancement during the ultrastructural assessment of cilia.

Ultrastruct Pathol

a Central EM-Lab, Department of Pathology , University Hospital Regensburg, Regensburg , Germany.

Published: June 2018

AI Article Synopsis

Article Abstract

Routine diagnostic electron microscopy of primary ciliary dyskinesia (PCD) is based on the findings of ultrastructural defects of axonemal components. Assessment of the typical abnormalities can be enhanced by improving the sample preservation status using tannic acid (TA) as additive in the biopsy fixation or processing steps. Another option is the implementation of computer-assisted image analysis tools. Advancements in high-resolution 3D visualization of the axonemal structure have been noted, with great potential for the future diagnosis of inherited cilia disorders.

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Source
http://dx.doi.org/10.1080/01913123.2017.1363335DOI Listing

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