Correction for 'Enzyme-responsive reporter molecules for selective localization and fluorescence imaging of pathogenic biofilms' by Junxin Aw et al., Chem. Commun., 2017, 53, 3330-3333.
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| http://dx.doi.org/10.1039/c7cc90354e | DOI Listing |
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Pooled PPIseq: Screening the SARS-CoV-2 and human interface with a scalable multiplexed protein-protein interaction assay platform.
PLoS One
January 2025
SLAC National Accelerator Laboratory, Stanford University, Stanford, California, United States of America.
Protein-Protein Interactions (PPIs) are a key interface between virus and host, and these interactions are important to both viral reprogramming of the host and to host restriction of viral infection. In particular, viral-host PPI networks can be used to further our understanding of the molecular mechanisms of tissue specificity, host range, and virulence. At higher scales, viral-host PPI screening could also be used to screen for small-molecule antivirals that interfere with essential viral-host interactions, or to explore how the PPI networks between interacting viral and host genomes co-evolve.
View Article and Find Full Text PDFQuick Freezing-Induced Au Nanoparticle Aggregates (QFIAAs) for Near-IR (NIR) Surface-Enhanced Raman Scattering (SERS) Substrates.
Langmuir
January 2025
Department of Chemistry, SUNY Buffalo State University, 1300 Elmwood Ave., Buffalo, New York 14222, United States.
Here, we report a simple method to prepare near-IR (NIR) surface-enhanced Raman scattering (SERS) substrates by quickly freezing a citrate-capped Au nanoparticle (AuNP) solution in liquid nitrogen, followed by thawing it at room temperature. This process aggregates AuNPs in a controlled manner by forming ice crystals with smaller grain sizes when compared to a slow freezing process. The resulting smaller AuNP aggregates remain suspended in solution long enough to conduct high-throughput chemical analysis in a microwell plate using the NIR SERS spectroscopy.
View Article and Find Full Text PDFEthosuximide: Subunit- and Gβγ-dependent blocker and reporter of allosteric changes in GIRK channels.
Br J Pharmacol
January 2025
Department of Physiology and Pharmacology, School of Medicine, Tel Aviv University, Tel Aviv, Israel.
Background And Purpose: The antiepileptic drug ethosuximide (ETX) suppresses epileptiform activity in a mouse model of GNB1 syndrome, caused by mutations in Gβ protein, likely through the inhibition of G-protein gated K (GIRK) channels. Here, we investigated the mechanism of ETX inhibition (block) of different GIRKs.
Experimental Approach: We studied ETX inhibition of GIRK channels expressed in Xenopus oocytes with or without their physiological activator, the G protein subunit dimer Gβγ.
Bioorthogonal chemical reporters for profiling retinoic acid-modified and retinoic acid-interacting proteins.
Bioorg Med Chem
January 2025
State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China. Electronic address:
Vitamin A and its primary active derivative, all-trans retinoic acid (RA), are endogenous signaling molecules essential for numerous biological processes, including cell proliferation, differentiation, and immune modulation. Owing to its differentiation-inducing effect, RA was the first differentiating agent approved for the clinical treatment of acute myeloid leukemia. While the classical mechanisms of RA signaling involve nuclear receptors, such as retinoic acid receptors (RARs), emerging evidence suggests that RA also engages in non-covalent and covalent interactions with a broader range of proteins.
View Article and Find Full Text PDFLayer-by-layer thin films of TiC MXene and gold nanoparticles as an ideal SERS platform.
Phys Chem Chem Phys
January 2025
Department of Chemical Engineering, Ataturk University, 25240 Erzurum, Turkey.
The combination of plasmonic metals and MXene, as a new and interesting member of the 2D material class, may provide unique advantages in terms of low cost, versatility, flexibility, and improved activity as an ideal surface-enhanced Raman spectroscopy (SERS) platform. Despite the recent progress, the present studies on the utilization of plasmonic metal/MXene-based SERS systems are quite limited and thereby benefits of the extraordinary properties of this combination cannot be realized. In this study, for the first time, we propose layer-by-layer (LbL) thin films of TiC MXene and gold nanoparticles (AuNPs) as a robust SERS platform (TiC/AuNPs).
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