Recent research has shown that the molecule c-di-GMP is an important second messenger regulating various functions in bacteria. In particular, the implication of c-di-GMP as a positive regulator of adhesion and biofilm formation has gained momentum as a highly relevant research topic, as detailed knowledge about the underlying regulatory mechanisms may enable the development of measures to control biofilms in both industrial and medical settings. Accordingly, it is in many cases of interest to measure the c-di-GMP level in bacteria under specific conditions or in specific mutant strains. We have developed a collection of fluorescence-based c-di-GMP biosensors capable of gauging the c-di-GMP level in Pseudomonas aeruginosa and closely related bacteria. Here, we describe protocols for the use of these biosensors in gauging and visualizing cellular c-di-GMP levels of P. aeruginosa both in in vitro setups such as continuous-culture flow-cell biofilms, and in in vivo settings such as a murine corneal infection model.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-7240-1_8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An Comparative Analysis of Pain Perception in Children Following Lidocaine Gel, Clove Oil, and Precooling for Intraoral Injections: A Pilot Study.
Int J Clin Pediatr Dent
December 2024
Department of Pediatric and Preventive Dentistry, Vinayaka Mission's Sankarachariyar Dental College, Vinayaka Mission's Research Foundation (Deemed to be University), Salem, Tamil Nadu, India.
Background: Dental anxiety about injections are common challenge in pediatric dentistry, often leading to delayed dental treatment.
Aim: The aim of the study was to evaluate the anesthetic effectiveness of three different topical agents in pediatric dental procedures.
Settings And Design: The study was a cross-sectional study carried out in the department of pediatric and preventive dentistry.
Twelve-Month Outcomes and Optical Coherence Tomography (OCT) Biomarkers After Intravitreal Dexamethasone Implantation in Pseudophakic Eyes with Post-Vitrectomy Cystoid Macular Edema (CME)-Refractory to Medical Therapy.
Diagnostics (Basel)
January 2025
Department of Ophthalmology, Policlinico Riuniti Foggia, University of Foggia, 71122 Foggia, Italy.
: In this study, we evaluated the incidence of cystoid macular edema (CME) after pars plana vitrectomy (PPV) for different retinal pathologies and assessed the role of optical coherence tomography (OCT) biomarkers in guiding treatment decisions in post-surgical CME patients who were refractory to medical therapy over a follow-up period of 12 months. : Medical records of consecutive pseudophakic patients, who underwent PPV for different retinal pathologies, were retrospectively evaluated in this single-center, uncontrolled study. The incidence of post-PPV CME was assessed.
View Article and Find Full Text PDFEfficiency and safety comparison between 20,000 and 10,000 cuts per minute vitrectomy using a 27G cutter in rhegmatogenous retinal detachment: a prospective randomized controlled study.
Jpn J Ophthalmol
January 2025
Department of Ophthalmology, Eye center, China Medical University Hospital, Taichung City, Taiwan.
Purpose: To compare the efficac and safety of a dual-blade 20,000 cuts per minute (cpm) vitrectomy probe with a single-blade 10,000 cpm probe for primary rhegmatogenous retinal detachment (RRD).
Study Design: Prospective, randomized controlled clinical trial.
Methods: Evaluations were conducted preoperatively, intraoperatively, and at three months postoperatively.
SIRE: Scale-invariant, rotation-equivariant estimation of artery orientations using graph neural networks.
Med Image Anal
January 2025
Department of Applied Mathematics, Technical Medical Centre, University of Twente, Drienerlolaan 5, 7522 NB Enschede, The Netherlands.
The orientation of a blood vessel as visualized in 3D medical images is an important descriptor of its geometry that can be used for centerline extraction and subsequent segmentation, labeling, and visualization. Blood vessels appear at multiple scales and levels of tortuosity, and determining the exact orientation of a vessel is a challenging problem. Recent works have used 3D convolutional neural networks (CNNs) for this purpose, but CNNs are sensitive to variations in vessel size and orientation.
View Article and Find Full Text PDFA Novel Custom Bent 27-Gauge Needle-Guided Suture Snare Technique for Scleral Fixation of Posterior Chamber Intraocular Lenses.
Retina
February 2025
Department of Ophthalmology, Liyang Hospital of Chinese Medicine, Liyang, China.
Purpose: To describe a custom bent 27-gauge needle-guided suture snare technique for scleral fixation of posterior chamber intraocular lenses (PCIOL).
Methods: An 8-0 polypropylene suture was threaded into the lumen of a custom bent 27-gauge needle, and the needle tip was advanced into the eye from the intraocular lens (IOL) fixation point. The suture was threaded through the posterior limbal incision inside the IOL haptic loop and pulled out.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!