AI Article Synopsis
- Recent advancements in genetic tools have been made for filamentous fungi, which are important in agriculture and food industries.
- Traditional gene targeting methods have been inefficient, limiting functional genomics studies in these organisms.
- The introduction of CRISPR/Cas9 technology offers a highly efficient and versatile method for genetic research in fungi, paving the way for better understanding and utilization of fungal biodiversity.
Article Abstract
In recent years, a variety of genetic tools have been developed and applied to various filamentous fungi, which are widely applied in agriculture and the food industry. However, the low efficiency of gene targeting has for many years hampered studies on functional genomics in this important group of microorganisms. The emergence of CRISPR/Cas9 genome-editing technology has sparked a revolution in genetic research due to its high efficiency, versatility, and easy operation and opened the door for the discovery and exploitation of many new natural products. Although the application of the CRISPR/Cas9 system in filamentous fungi is still in its infancy compared to its common use in E. coli, yeasts, and mammals, the deep development of this system will certainly drive the exploitation of fungal diversity. In this review, we summarize the research progress on CRISPR/Cas9 systems in filamentous fungi and finally highlight further prospects in this area.
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| http://dx.doi.org/10.1007/s00253-017-8497-9 | DOI Listing |
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