AI Article Synopsis
- The study focused on creating accurate cancer models using ultrasound for implanting both neuroblastoma (NB) and Ewing's sarcoma (ES) cells in specific organs (adrenal gland for NB, renal subcapsular for ES).
- Researchers injected various NB cell lines and patient-derived tumors, as well as ES cells, and tracked tumor progression by in vivo imaging.
- They found that the tumors developed locally and metastasized to several organs within 5 weeks, retaining features of the original cancers, indicating that ultrasound implantation is a promising method for preclinical cancer research.
Article Abstract
Background: Advances in cancer therapeutics depend on reliable in vivo model systems. To develop biologically relevant xenografts, ultrasound was utilized for tissue-directed implantation of neuroblastoma (NB) cell line and patient-derived tumors in the adrenal gland, and for renal subcapsular engraftment of Ewing's sarcoma (ES).
Materials And Methods: NB xenografts were established by direct adrenal injection of luciferase-transfected NB cell lines (IMR32, SH-SY5Y, SK-N-BE2) or NB patient-derived tumor cells (UMNBL001, UMNBL002). ES xenografts were established by renal subcapsular injection of TC32, A673, CHLA-25, or A4573 cells. Progression was monitored by in vivo imaging.
Results: Tumors progressed to local disease with metastasis evident by 5 weeks. Metastatic sites included cortical bone, lung, liver, and lymph nodes. Xenografted tumors retained immunochemical features of the original cancer.
Conclusion: Human NB adrenal xenografts, including two patient-derived orthotopic, and ES renal subcapsular xenografts were established by ultrasound without open surgery. Tissue-directed implantation is an effective technique for developing metastatic preclinical models.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656850 | PMC |
| http://dx.doi.org/10.21873/invivo.11131 | DOI Listing |
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