To investigate the cellular response to designed topography in vitro, we studied the adhesion, proliferation, osteogenic differentiation, and calcification of mouse preosteoblasts (MC3T3-E1) cultured on titanium (Ti) surfaces with periodic micrometer scale grooves containing nanometer scale ripples in the vertical direction fabricated by single-shot, femtosecond laser irradiation (fsTi). The surface composition and chemical state of fsTi were almost the same as those of mirror-polished Ti without femtosecond laser irradiation (mTi). Cells cultured on fsTi were highly aligned, whereas the cell proliferation rate on fsTi was less than that on mTi. Higher gene expressions of Spp1 and Bglap1 were detected in cells cultured on fsTi than those on mTi, indicating that the periodic micro/nanometer scale grooves topography promoted osteogenic differentiation and calcification. This initial activation of osteoinduction on fsTi generated calcified deposits that were thicker and larger than those on mTi and hence, osteoconductivity was promoted on fsTi. Our findings indicate that femtosecond laser irradiation is a technique with potential for controlling biomaterial-cell interfaces and, in particular, the promotion of osseointegration of Ti. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3456-3464, 2017.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jbm.a.36202 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Investigating the effect of femtosecond laser pulses on noscapine and curcumin cellular uptake and improving drug delivery in gastric cancer cells.
Heliyon
January 2025
Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran.
The MKN45 cell line, a type of gastric cancer cell, exhibits resistance to chemotherapy agents through various mechanisms. Curcumin and noscapine, two plant-derived anticancer compounds, exhibit selective cytotoxicity towards cancer cells. However, their bioavailability is poor both in vitro and in vivo.
View Article and Find Full Text PDF3D Finite Element Modeling of Femtosecond Laser Trabeculotomy.
Lasers Surg Med
January 2025
ViaLase Inc., Aliso Viejo, California, USA.
Objectives: Femtosecond laser image guided high precision trabeculotomy (FLigHT) is a novel open-angle glaucoma treatment. The procedure non-invasively creates aqueous humor (AH) drainage channel from the anterior chamber (AC) to Schlemm's canal (SC) through the trabecular meshwork (TM) to decrease intraocular pressure (IOP). The purpose of this study was to develop a 3D finite element model (FEM) of the FLigHT procedure and to simulate clinical results for different drainage channel cross-sectional areas.
View Article and Find Full Text PDFBonding efficacy of highly translucent zirconia and lithium disilicate glass ceramics after femtosecond laser irradiation.
J Prosthodont Res
January 2025
Department of Gerodontology and Oral Rehabilitation, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Purpose: This study investigated the effects of femtosecond laser (FL) irradiation on the surface roughness and shear bond strength of high-translucency zirconia (6 mol% yttria-partially stabilized zirconia [6Y-PSZ]) and lithium disilicate (LiSiO) glass ceramics.
Methods: Fully sintered square-shaped specimens of 6Y-PSZ (7 groups; 20 specimens/group) and LiSiO (8 groups; 20 specimens/group) were surface-treated via sandblasting (50-μm alumina sand or glass beads) or FL irradiation (20- or 40-μm dot or cross-line patterns) or using Monobond Etch & Prime (Ivoclar Vivadent AG; only for LiSiO specimens). The surface roughness (arithmetic average [Sa] and developed interfacial area ratio [Sdr]) and shear bond strength after 24 h and 10,000 thermal cycles were measured and statistically analyzed.
Adaptable electro-optic detection of THz radiation using a laser-written bull's-eye antenna.
Sci Rep
January 2025
Department of Physics, University of Ottawa, Ottawa, ON, K1N 6N5, Canada.
We report a nonlinear terahertz (THz) detection device based on a metallic bull's-eye plasmonic antenna. The antenna, fabricated with femtosecond laser direct writing and deposited on a nonlinear gallium phosphide (GaP) crystal, focuses incoming THz waveforms within the sub-wavelength bull's eye region to locally enhance the THz field. Additionally, the plasmonic structure minimizes diffraction effects allowing a relatively long interaction length between the transmitted THz field and the co-propagating near-infrared gating pulse used in an electro-optic sampling configuration.
View Article and Find Full Text PDFSupercontinuum generation in scintillator crystals.
Sci Rep
January 2025
Laser Research Center, Vilnius University, Saulėtekio Avenue 10, LT-10223, Vilnius, Lithuania.
We present a comparative experimental study of supercontinuum generation in undoped scintillator crystals: bismuth germanate (BGO), yttrium orthosilicate (YSO), lutetium oxyorthosilicate (LSO), lutetium yttrium oxyorthosilicate (LYSO) and gadolinium gallium garnet (GGG), pumped by 180 fs fundamental harmonic pulses of an amplified Yb:KGW laser. In addition to these materials, experiments in yttrium aluminium garnet (YAG), potassium gadolinium tungstate (KGW) and lithium tantalate (LT) were performed under identical experimental settings (focusing geometry and sample thickness), which served for straightforward comparison of supercontinuum generation performances. The threshold and optimal (that produces optimized red-shifted spectral extent) pump pulse energies for supercontinuum generation were evaluated from detailed measurements of spectral broadening dynamics.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!