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Utilization of Stable and Efficient High-Entropy (NiZnMgCuCo)AlO Catalyst with Polyvalent Transition Metals to Boost Peroxymonosulfate Activation toward Pollutant Degradation.
Small
January 2025
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.
A polyacrylamide gel method has been used to synthesize a variety of polyvalent-transition-metal-doped Ni position of high entropy spinel oxides (NiZnMgCuCo)AlO-800 °C (A) on the basis of NiAlO, and the catalytic activity of A is studied under the synergistic action of peroxymonosulfate (PMS) activation and simulated sunlight. The A containing polyvalent transition metals (Ni, Cu, and Co) can effectively activate PMS and efficiently degrade levofloxacin (LEV) and tetracycline hydrochloride (TCH) under simulated sunlight irradiation. After 90 min of light exposure, the degradation percentages of LEV (50 mg L) and TCH (100 mg L) degrade by the A/PMS/vis system reach 87.
View Article and Find Full Text PDFGel-Based Sample Fractionation with SP3-Purification for Top-Down Proteomics.
J Proteome Res
January 2025
Advanced Research Support Center, Ehime University, Ehime 791-0295, Japan.
Precise prefractionation of proteome samples is a potent method for realizing in-depth analysis in top-down proteomics. PEPPI-MS (Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS), a gel-based sample fractionation method, enables high-resolution proteome fractionation based on molecular weight by highly efficient extraction of proteins from polyacrylamide gels after SDS-PAGE separation. Thereafter it is essential to effectively remove contaminants such as CBB and SDS from the PEPPI fraction prior to mass spectrometry.
View Article and Find Full Text PDFPurification and expression of a novel bacteriocin, JUQZ-1, against pv. (PSA), secreted by Wq-1, isolated from the rhizosphere soil of healthy kiwifruit.
Front Microbiol
January 2025
College of Biology Resources and Environmental Sciences, Jishou University, Jishou, China.
Kiwifruit canker, caused by pv. (PSA), has led to significant losses in the kiwifruit industry each year. Due to the drug resistance feature of PSA, biological control is currently the most promising method.
View Article and Find Full Text PDFImproving protein hydrolysis and digestibility in biomass through recombinant peptidases (EC 3.4): Opportunities for monogastric animal diets.
Heliyon
January 2025
CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477, Lisboa, Portugal.
This study investigates the use of recombinant peptidases (EC 3.4) to improve protein hydrolysis and digestibility in , with a focus on addressing the challenge of reduced protein bioavailability for monogastric animals due to resistant protein-pigment formations, such as phycocyanin, and increased digesta viscosity caused by jellification properties. A library of 192 peptidases was generated, from which 142 soluble peptidases were expressed in and subsequently screened for activity against an suspension .
View Article and Find Full Text PDFSimultaneous extraction and purification of polysaccharides and proteins from Pleurotus ostreatus using an aqueous two-phase system.
J Food Sci
January 2025
College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, China.
Pleurotus ostreatus is a nutrient-dense edible fungus renowned for its delicate texture, appealing flavor, and numerous potential health benefits. Simultaneous extraction within the framework of food resource processing facilitates the concurrent isolation and analysis of multiple target compounds. In this study, an ethanol/salt aqueous two-phase system (ATPS) was employed to extract polysaccharides (PS) and proteins from P.
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