AI Article Synopsis
- RasGRP3 is found to be overexpressed in papillary thyroid cancer (PTC) tissues and cell lines, suggesting it plays a significant role in the disease.
- Downregulation of RasGRP3 via small interfering RNA reduces cell proliferation and migration, as well as tumor growth, indicating its oncogenic properties.
- The study reveals that RasGRP3 influences PTC progression through the activation of MDM2 via the Akt signaling pathway, highlighting its potential as a therapeutic target.
Article Abstract
Accumulating evidence has shown that Ras guanylnucleotide releasing peptide 3 (RasGRP3) is up-regulated in several distinct cancer types; however, its role in papillary thyroid cancer (PTC) remains unclear. In this study, we demonstrate that RasGRP3 was overexpressed in PTC tissues and cell lines. Downregulation of RasGRP3 using small interfering (si) RNA significantly inhibited PTC cell proliferation and migration in vitro, and tumor growth in vivo, reflecting an oncogenic role of RasGRP3 in PTC. We subsequently identified that the expression of mouse double minute 2 homolog (MDM2) and phosphorylated Akt (p-Akt) was significantly decreased in RasGRP3-downregulated PTC cells. Overexpression of MDM2 attenuated the function of si-RasGRP3. Taken together, our data show that RasGRP3 exerts its oncogenic effect in PTC through Akt-mediated MDM2 activation. RasGRP3 may serve as a potential new therapeutic target for PTC.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.gene.2017.08.024 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Poly (ADP-Ribose) Polymerase 1 Induces Cyclic GMP-AMP Synthase-stimulator of Interferon Genes Pathway Dysregulation to Promote Immune Escape of Colorectal Cancer Cells.
J Immunother
October 2024
Department of Radiation Oncology, Huai'an Hospital Affiliated to Xuzhou Medical University, Huai'an, China.
Colorectal cancer (CRC) ranks third globally in cancer incidence and mortality, posing a significant human concern. Recent advancements in immunotherapy are noteworthy. This study explores immune modulation for CRC treatment.
View Article and Find Full Text PDFIn vivo selection of hepatocytes.
Hepatology
October 2024
Department of Pediatrics, Papé Family Pediatric Research Institute, Oregon Health & Science University, Portland, Oregon, USA.
The liver is a highly regenerative organ capable of significant proliferation and remodeling during homeostasis and injury responses. Experiments of nature in rare genetic diseases have illustrated that healthy hepatocytes may have a selective advantage, outcompete diseased cells, and result in extensive liver replacement. This observation has given rise to the concept of therapeutic liver repopulation by providing an engineered selective advantage to a subpopulation of beneficial hepatocytes.
View Article and Find Full Text PDFMicroSphere 3D Structures Delay Tissue Senescence through Mechanotransduction.
ACS Nano
January 2025
Department of Orthopedics, The First Affiliated Hospital of Soochow University, 899 Pinghai Road, Soochow, Jiangsu 215000, China.
The extracellular matrix (ECM) stores signaling molecules and facilitates mechanical and biochemical signaling in cells. However, the influence of biomimetic "rejuvenation" ECM structures on aging- and degeneration-related cellular activities and tissue repair is not well understood. We combined physical extrusion and precise "on-off" alternating cross-linking methods to create anisotropic biomaterial microgels (MicroRod and MicroSphere) and explored how they regulate the cell activities of the nucleus pulposus (NP) and their potential antidegenerative effects on intervertebral discs.
View Article and Find Full Text PDFPlasmacytoid dendritic cell proliferation and acute myeloid leukemia with minimal differentiation (AML-M0).
Clin Chem Lab Med
January 2025
Clinical Laboratory, Sir Run Run Shaw Hospital, School of Medicine, 56660 Zhejiang University, Hangzhou, Zhejiang, China.
The role of lin-12 notch in C. elegans anchor cell proliferation.
Biol Open
December 2024
Department of Dermatology, University of Zurich, University Hospital Zurich, Schlieren CH-8952, Switzerland.
The gonadal anchor cell (AC) is an essential organizer for the development of the egg-laying organ in the C. elegans hermaphrodite. Recent work has investigated the mechanisms that control the quiescent state the AC adopts while fulfilling its functions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!